AJP - Endo AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab (August 6, 2002). doi:10.1152/ajpendo.00116.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/E1167    most recent
00116.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fang, X.
Right arrow Articles by Mitchell, B. F
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fang, X.
Right arrow Articles by Mitchell, B. F

Articles in PresS, published online ahead of print August 6, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00116.2002
Submitted on March 14, 2002
Accepted on July 31, 2002

Messenger RNA for Progesterone Receptor Isoforms in the Late Gestation Rat Uterus

Xin Fang1, Susan Wong1, and Bryan F Mitchell1*

1 Department of Obstetrics and Gynecology, University of Alberta, Perinatal Research Centre, Edmonton, Alberta, Canada

* To whom correspondence should be addressed. E-mail: brymitch{at}ualberta.ca.

The progesterone receptor (PR) has three isoforms, PR-A, PR-B and PR-C, which have different physiologic effects. PR-A may inhibit PR-B-mediated transcription. Parturition requires withdrawal of progesterone (P4). This could occur through decreased P4 concentrations and/or a change in PR isoforms to diminish the effect of P4. We measured mRNA for PR isoforms in rat uterine tissues through late gestation and investigated the effects of antagonists to estrogen (tamoxifen) and P4 (RU486). Two specific probes were used for ribonuclease protection assays - one (PR-total) measured PR-A, PR-B and PR-C and the other recognized only PR-B. PR-total mRNA increased significantly through late gestation whereas PR-B was unchanged. The ratio of PR-total to PR-B peaked the day prior to parturition. Tamoxifen delayed parturition and inhibited the increase in PR-total without affecting PR-B mRNA. RU486 caused early parturition associated with increased PR-total mRNA with no change in PR-B. We conclude there are significant changes in PR isoforms in late gestation rat uterus. These changes may be regulated by estrogen and P4 and may influence the timing of parturition.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. F. Mitchell and M. J. Taggart
Are animal models relevant to key aspects of human parturition?
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2009; 297(3): R525 - R545.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. F. Mitchell, B. Zielnik, S. Wong, C. D. Roberts, and J. M. Mitchell
Intraperitoneal infusion of proinflammatory cytokines does not cause activation of the rat uterus during late gestation
Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E658 - E664.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.