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1 Dept. of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, The Copenhagen Muscle Research Centre, DK-2100 Copenhagen O, Denmark
2 Dept. of Medical Physiology, Panum Institute, University of Copenhagen, The Copenhagen Muscle Research Centre, DK-2200 Copenhagen N, Denmark
3 Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
4 Dept. of Molecular Muscle Biology, Rigshospitalet, The Copenhagen Muscle Research Centre, DK-2200 Copenhagen N, Denmark; Dept. of Sport Science, University of Aarhus, DK-8200 Aarhus, Denmark
5 Dept. of Molecular Muscle Biology, Rigshospitalet, The Copenhagen Muscle Research Centre, DK-2200 Copenhagen N, Denmark
6 Dept. of Rheumatology, Bispebjerg Hospital, The Copenhagen Muscle Research Centre, DK-2400 Copenhagen NV, Denmark
* To whom correspondence should be addressed. E-mail: croepstorff{at}ifi.ku.dk.
Women have been shown to use more intramuscular triacylglycerol (IMTG) during exercise compared with men. To investigate whether this could be due to gender-specific regulation of HSL and to use gender-comparison as a model to gain further insight into HSL regulation, nine women and eight men performed bicycle exercise (90 min, 60% VO2 peak), and skeletal muscle HSL expression, phosphorylation, and activity were determined. Supporting previous findings, basal IMTG content (p<0.001) and net IMTG decrease during exercise (p<0.01) were higher in women than in men and correlated significantly (r=0.72, p=0.001). Muscle HSL mRNA (80%, p=0.11) and protein (50%, p<0.05) content were higher in women than in men. HSL total activity increased during exercise (47%, p<0.05) but did not differ between genders (NS). Accordingly, HSL specific activity (HSL activity per HSL protein content) increased during exercise (62%, p<0.05) and was generally higher in men than in women (82%, p<0.05). A similar pattern was observed for HSL Ser659 phosphorylation, suggesting a role for HSL Ser659 in regulation of HSL activity. Likewise, plasma epinephrine increased during exercise (p<0.05) and was higher in men than in women during the final part of the exercise bout (p<0.05). It is concluded that although HSL expression and Ser659 phosphorylation in skeletal muscle during exercise is gender specific, total muscle HSL activity measured in vitro was similar in the two sexes. The higher basal IMTG content in women compared with men is therefore the best candidate to explain the higher IMTG net hydrolysis during exercise in women.
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