| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print May 28, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00092.2002
Submitted on March 1, 2002
Accepted on April 1, 2002
1 Biochemistry, Boston University School of Medicine, Boston, MA, USA
* To whom correspondence should be addressed. E-mail: ppilch{at}bu.edu.
Insulin regulates the uptake of glucose into skeletal muscle and adipocytes by redistributing the tissue-specific glucose transporter, Glut4, from intracellular vesicles to the cell surface. To date, Glut4 is the only protein involved in insulin-regulated vesicular traffic that has this tissue distribution, thus raising the possibility that its expression alone may allow formation of an insulin responsive vesicular compartment. We show here that treatment of differentiating C2C12 myoblasts with dexamethasone, acting via the glucocorticoid receptor, causes at least a 10 fold increase in Glut4 expression but results in no significant change in insulin stimulated glucose transport. Signaling from the insulin receptor to its target, Akt2, and expression of the SNARE proteins, syntaxin 4 and VAMP2, are normal in dexamethasone treated C2C12 cells. However, these cells show no insulin-dependent trafficking of the insulin responsive aminopeptidase (IRAP) or the transferrin receptor, respective markers for intracellular Glut4-rich compartments and endosomes that are insulin responsive in mature muscle and adipose cells. Therefore, these data support the hypothesis that Glut4 expression, itself, is insufficient to establish an insulin sensitive vesicular compartment.
This article has been cited by other articles:
|
|
 |
|
  P. P. H. Hommelberg, J. Plat, R. C. J. Langen, A. M. W. J. Schols, and R. P. Mensink Fatty acid-induced NF-{kappa}B activation and insulin resistance in skeletal muscle are chain length dependent Am J Physiol Endocrinol Metab, January 1, 2009; 296(1): E114 - E120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Park, T. L. Scheffler, A. M. Gunawan, H. Shi, C. Zeng, K. M. Hannon, A. L. Grant, and D. E. Gerrard Chronic elevated calcium blocks AMPK-induced GLUT-4 expression in skeletal muscle Am J Physiol Cell Physiol, January 1, 2009; 296(1): C106 - C115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Nieto-Vazquez, S. Fernandez-Veledo, C. de Alvaro, and M. Lorenzo Dual Role of Interleukin-6 in Regulating Insulin Sensitivity in Murine Skeletal Muscle Diabetes, December 1, 2008; 57(12): 3211 - 3221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Pooley, K. L. Moynihan, V. Soukoulis, S. Reddy, R. Francis, C. Lo, L.-J. Ma, and D. M. Bader Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport J. Cell Sci., October 15, 2008; 121(20): 3413 - 3421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lorenzo, S. Fernandez-Veledo, R. Vila-Bedmar, L. Garcia-Guerra, C. De Alvaro, and I. Nieto-Vazquez Insulin resistance induced by tumor necrosis factor-{alpha} in myocytes and brown adipocytes J Anim Sci, April 1, 2008; 86(14_suppl): E94 - E104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nedachi and M. Kanzaki Regulation of glucose transporters by insulin and extracellular glucose in C2C12 myotubes Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E817 - E828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Sultan, B. Henkel, M. Terlou, and H. P. Haagsman Quantification of hormone-induced atrophy of large myotubes from C2C12 and L6 cells: atrophy-inducible and atrophy-resistant C2C12 myotubes Am J Physiol Cell Physiol, February 1, 2006; 290(2): C650 - C659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Smith, P. B. Patil, S. D. Minteer, J. R. Lipsitz, and J. S. Fisher Possibility of Autocrine {beta}-Adrenergic Signaling in C2C12 Myotubes Experimental Biology and Medicine, December 1, 2005; 230(11): 845 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Smith, P. B. Patil, and J. S. Fisher AICAR and hyperosmotic stress increase insulin-stimulated glucose transport J Appl Physiol, September 1, 2005; 99(3): 877 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. Kase, A. J. Wensaas, V. Aas, K. Hojlund, K. Levin, G. H. Thoresen, H. Beck-Nielsen, A. C. Rustan, and M. Gaster Skeletal Muscle Lipid Accumulation in Type 2 Diabetes May Involve the Liver X Receptor Pathway Diabetes, April 1, 2005; 54(4): 1108 - 1115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. R. Schlaepfer, L. K. Pulawa, L. D. M. C-B. Ferreira, D. E. James, W. H. Capell, and R. H. Eckel Increased expression of the SNARE accessory protein Munc18c in lipid-mediated insulin resistance J. Lipid Res., June 1, 2003; 44(6): 1174 - 1181. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
