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Articles in PresS, published online ahead of print June 11, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00053.2002
Submitted on February 6, 2002
Accepted on June 3, 2002
1 Surgery, University of Texas Medical Branch, Galveston, TX, USA; Metabolism, Shriners Hospitals for Children, Galveston, TX, USA
* To whom correspondence should be addressed. E-mail: rwolfe{at}utmb.edu.
We have developed a new method to determine the fractional synthesis rate (FSR) and breakdown rate (FBR) of muscle protein. This method involves a pulse tracer injection and measurement of enrichment in the arterial blood and muscle at three time points. The calculations of FSR and FBR are based on the precursor-product principle. To test this method we gave pulse injection of L-[ring-13C6]phenylalanine of 4-6 mg/kg in 5 rabbits. The measured FBR value (0.233 ± 0.060%/h) was almost identical (p = 0.35) to that (0.217 ± 0.078%/h) estimated from a leg arteriovenous balance model (Biolo et al, JPEN 16:305-315, 1992). The measured FSR value tended to be lower than that estimated from the leg model (0.125 ± 0.036 vs. 0.185 ± 0.086%/h; p = 0.14), possibly because the new method measures only muscle FSR, whereas the leg balance model also includes skin and bone contributions. The pulse tracer injection did not affect muscle protein kinetics as measured by leucine and phenylalanine kinetics in the leg. In another 5 rabbits we demonstrated that sampling could be reduced to either 1 or 2 muscle biopsies when multiple pulse injections were used. This method can be completed in one hour with one muscle biopsy, and has technical advantages over currently used methods.
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