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1 Kinesiology, University of Michigan, Ann Arbor, Michigan, United States
* To whom correspondence should be addressed. E-mail: jeffhoro{at}umich.edu.
Although the increase in fatty acid oxidation after endurance exercise training has been linked with improvements in insulin sensitivity and overall metabolic health, the mechanisms responsible for increasing fatty acid oxidation after exercise training are not completely understood. The primary aim of this study was to determine the effect of adding endurance exercise training to a weight-loss program on fat oxidation and the co-localization of the fatty acid translocase, FAT/CD36, with carnitine palmitoyl transferase-I (CPT-I) in human skeletal muscle. We measured post-absorptive fat oxidation and acquired a muscle sample from abdominally obese women before and after 12% body-weight-loss through either dietary intervention with endurance exercise training (EX+DIET) or dietary intervention without endurance exercise training (DIET). Immunoprecipitation techniques were used on these muscle samples to determine if the association between FAT/CD36 and CPT-I is altered after DIET and/or EX+DIET. FAT/CD36 was found to co-immunoprecipitate with CPT-I and the amount of FAT/CD36 that co-immunoprecipitated with CPT-I increased by ~25% after EX+DIET (P<0.005), but was unchanged after DIET. In addition, the increase in the amount of FAT/CD36 that co-immunoprecipitated with CPT-I in EX+DIET was strongly correlated with the increase in whole body fat oxidation (R2=0.857, P<0.003). In conclusion, the findings from this study indicate that exercise training alters the localization of FAT/CD36 and increases its association with CPT-I, which may help augment fat oxidation.
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