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Articles in PresS, published online ahead of print June 4, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00040.2002
Submitted on January 31, 2002
Accepted on May 23, 2002
1 Department of Medicine and Bioregulatory Science, Graduate School of Medical Siences, Kyushu University, Fukuoka, Fukuoka, Japan
2 Department of Immunobiology and Neuroscience, Division of Clinical Immunology, Medical Institute of Bioregulation, Kyushu University, Beppu, Ohita, Japan
* To whom correspondence should be addressed. E-mail: toyoshi{at}intmed3.med.kyushu-u.ac.jp.
Adipose differentiation-related protein (ADRP) is a lipid droplet associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation.
In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed that the number and size of lipid droplets apparently increased comparing to those of control cells. Overexpressed GFP-ADRP mainly located at the surface of lipid droplets and appeared like "ring-shaped". Triacylglycerol content was also significantly (p<0.001) increased in GFP-ADRP overexpressed cells comparing to the control cells. ADRP induced-lipid accumulation did not depend on adipocyte-specific genes induction such as peroxisome proliferator-activated receptor-
(PPAR ), lipoprotein lipase (LPL) or other lipogenic genes including acyl-coenzymeA synthetase (ACS), fatty acid binding protein (FABP) and fatty acid transporter (FAT). In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remained to be elucidated.
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