| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print June 4, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00040.2002
Submitted on January 31, 2002
Accepted on May 23, 2002
1 Department of Medicine and Bioregulatory Science, Graduate School of Medical Siences, Kyushu University, Fukuoka, Fukuoka, Japan
2 Department of Immunobiology and Neuroscience, Division of Clinical Immunology, Medical Institute of Bioregulation, Kyushu University, Beppu, Ohita, Japan
* To whom correspondence should be addressed. E-mail: toyoshi{at}intmed3.med.kyushu-u.ac.jp.
Adipose differentiation-related protein (ADRP) is a lipid droplet associated protein that is expressed early during adipose differentiation. The present study was undertaken to reveal the role of ADRP in adipose differentiation.
In murine fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion protein expression adenovirus vector, confocal microscopic analysis showed that the number and size of lipid droplets apparently increased comparing to those of control cells. Overexpressed GFP-ADRP mainly located at the surface of lipid droplets and appeared like "ring-shaped". Triacylglycerol content was also significantly (p<0.001) increased in GFP-ADRP overexpressed cells comparing to the control cells. ADRP induced-lipid accumulation did not depend on adipocyte-specific genes induction such as peroxisome proliferator-activated receptor-
(PPAR ), lipoprotein lipase (LPL) or other lipogenic genes including acyl-coenzymeA synthetase (ACS), fatty acid binding protein (FABP) and fatty acid transporter (FAT). In conclusion, ADRP stimulated lipid accumulation and lipid droplet formation without induction of other adipocyte-specific genes or other lipogenic genes in murine fibroblasts. The detailed molecular mechanisms of ADRP on lipid accumulation remained to be elucidated.
This article has been cited by other articles:
|
|
 |
|
  E. B. Rankin, J. Rha, M. A. Selak, T. L. Unger, B. Keith, Q. Liu, and V. H. Haase Hypoxia-Inducible Factor 2 Regulates Hepatic Lipid Metabolism Mol. Cell. Biol., August 15, 2009; 29(16): 4527 - 4538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Sapiro, M. T. Mashek, A. S. Greenberg, and D. G. Mashek Hepatic triacylglycerol hydrolysis regulates peroxisome proliferator-activated receptor {alpha} activity J. Lipid Res., August 1, 2009; 50(8): 1621 - 1629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Fan, S. Ikuyama, J.-Q. Gu, P. Wei, J.-i. Oyama, T. Inoguchi, and J. Nishimura Oleic acid-induced ADRP expression requires both AP-1 and PPAR response elements, and is reduced by Pycnogenol through mRNA degradation in NMuLi liver cells Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E112 - E123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Q. Gu, S. Ikuyama, P. Wei, B. Fan, J.-i. Oyama, T. Inoguchi, and J. Nishimura Pycnogenol, an extract from French maritime pine, suppresses Toll-like receptor 4-mediated expression of adipose differentiation-related protein in macrophages Am J Physiol Endocrinol Metab, December 1, 2008; 295(6): E1390 - E1400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Anderson and J. Borlak Molecular Mechanisms and Therapeutic Targets in Steatosis and Steatohepatitis Pharmacol. Rev., September 1, 2008; 60(3): 311 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Varela, D. A. Antwi, R. Dhir, X. Yin, N. S. Singhal, M. J. Graham, R. M. Crooke, and R. S. Ahima Inhibition of ADRP prevents diet-induced insulin resistance Am J Physiol Gastrointest Liver Physiol, September 1, 2008; 295(3): G621 - G628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Paul, B. H.-J. Chang, L. Li, V. K. Yechoor, and L. Chan Deficiency of Adipose Differentiation-Related Protein Impairs Foam Cell Formation and Protects Against Atherosclerosis Circ. Res., June 20, 2008; 102(12): 1492 - 1501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Listenberger, A. G. Ostermeyer-Fay, E. B. Goldberg, W. J. Brown, and D. A. Brown Adipocyte differentiation-related protein reduces the lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover J. Lipid Res., December 1, 2007; 48(12): 2751 - 2761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Brasaemle Thematic review series: Adipocyte Biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis J. Lipid Res., December 1, 2007; 48(12): 2547 - 2559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Kotokorpi, E. Ellis, P. Parini, L.-M. Nilsson, S. Strom, K. R. Steffensen, J.-A. Gustafsson, and A. Mode Physiological Differences between Human and Rat Primary Hepatocytes in Response to Liver X Receptor Activation by 3-[3-[N-(2-Chloro-3-trifluoromethylbenzyl)-(2,2-diphenylethyl)amino]propyloxy]phenylacetic Acid Hydrochloride (GW3965) Mol. Pharmacol., October 1, 2007; 72(4): 947 - 955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. D. Russell, C. A. Palmer, D. J. Orlicky, A. Fischer, M. C. Rudolph, M. C. Neville, and J. L. McManaman Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism J. Lipid Res., July 1, 2007; 48(7): 1463 - 1475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nagayama, T. Uchida, and K. Gohara Temporal and spatial variations of lipid droplets during adipocyte division and differentiation J. Lipid Res., January 1, 2007; 48(1): 9 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Wolins, B. K. Quaynor, J. R. Skinner, A. Tzekov, M. A. Croce, M. C. Gropler, V. Varma, A. Yao-Borengasser, N. Rasouli, P. A. Kern, et al. OXPAT/PAT-1 Is a PPAR-Induced Lipid Droplet Protein That Promotes Fatty Acid Utilization Diabetes, December 1, 2006; 55(12): 3418 - 3428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bostrom, B. Magnusson, P.-A. Svensson, O. Wiklund, J. Boren, L. M. S. Carlsson, M. Stahlman, S.-O. Olofsson, and L. M. Hulten Hypoxia Converts Human Macrophages Into Triglyceride-Loaded Foam Cells Arterioscler Thromb Vasc Biol, August 1, 2006; 26(8): 1871 - 1876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Magnusson, L. Asp, P. Bostrom, M. Ruiz, P. Stillemark-Billton, D. Linden, J. Boren, and S.-O. Olofsson Adipocyte Differentiation-Related Protein Promotes Fatty Acid Storage in Cytosolic Triglycerides and Inhibits Secretion of Very Low-Density Lipoproteins Arterioscler Thromb Vasc Biol, July 1, 2006; 26(7): 1566 - 1571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Dalen, S. M. Ulven, B. M. Arntsen, K. Solaas, and H. I. Nebb PPAR{alpha} activators and fasting induce the expression of adipose differentiation-related protein in liver J. Lipid Res., May 1, 2006; 47(5): 931 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. R. Tobin, N. K. Harsem, K. T. Dalen, A. C. Staff, H. I. Nebb, and A. K. Duttaroy Regulation of ADRP expression by long-chain polyunsaturated fatty acids in BeWo cells, a human placental choriocarcinoma cell line J. Lipid Res., April 1, 2006; 47(4): 815 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H.-J. Chang, L. Li, A. Paul, S. Taniguchi, V. Nannegari, W. C. Heird, and L. Chan Protection against Fatty Liver but Normal Adipogenesis in Mice Lacking Adipose Differentiation-Related Protein Mol. Cell. Biol., February 1, 2006; 26(3): 1063 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Edvardsson, A. Ljungberg, D. Linden, L. William-Olsson, H. Peilot-Sjogren, A. Ahnmark, and J. Oscarsson PPAR{alpha} activation increases triglyceride mass and adipose differentiation-related protein in hepatocytes J. Lipid Res., February 1, 2006; 47(2): 329 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Masuda, H. Itabe, M. Odaki, K. Hama, Y. Fujimoto, M. Mori, N. Sasabe, J. Aoki, H. Arai, and T. Takano ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells J. Lipid Res., January 1, 2006; 47(1): 87 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Wei, S. Taniguchi, Y. Sakai, M. Imamura, T. Inoguchi, H. Nawata, S. Oda, Y. Nakabeppu, J. Nishimura, and S. Ikuyama Expression of Adipose Differentiation-Related Protein (ADRP) Is Conjointly Regulated by PU.1 and AP-1 in Macrophages J. Biochem., October 1, 2005; 138(4): 399 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bostrom, M. Rutberg, J. Ericsson, P. Holmdahl, L. Andersson, M. A. Frohman, J. Boren, and S.-O. Olofsson Cytosolic Lipid Droplets Increase in Size by Microtubule-Dependent Complex Formation Arterioscler Thromb Vasc Biol, September 1, 2005; 25(9): 1945 - 1951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Robenek, M. J. Robenek, I. Buers, S. Lorkowski, O. Hofnagel, D. Troyer, and N. J. Severs Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains J. Biol. Chem., July 15, 2005; 280(28): 26330 - 26338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ozeki, J. Cheng, K. Tauchi-Sato, N. Hatano, H. Taniguchi, and T. Fujimoto Rab18 localizes to lipid droplets and induces their close apposition to the endoplasmic reticulum-derived membrane J. Cell Sci., June 15, 2005; 118(12): 2601 - 2611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chung, J. M. Brown, M. B. Sandberg, and M. McIntosh Trans-10,cis-12 CLA increases adipocyte lipolysis and alters lipid droplet-associated proteins: role of mTOR and ERK signaling J. Lipid Res., May 1, 2005; 46(5): 885 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Larigauderie, C. Furman, M. Jaye, C. Lasselin, C. Copin, J.-C. Fruchart, G. Castro, and M. Rouis Adipophilin Enhances Lipid Accumulation and Prevents Lipid Efflux From THP-1 Macrophages: Potential Role in Atherogenesis Arterioscler Thromb Vasc Biol, March 1, 2004; 24(3): 504 - 510. [Abstract] [Full Text]
|
|
|
|
|
|
C.-J. Hu, L.-Y. Wang, L. A. Chodosh, B. Keith, and M. C. Simon Differential Roles of Hypoxia-Inducible Factor 1{alpha} (HIF-1{alpha}) and HIF-2{alpha} in Hypoxic Gene Regulation Mol. Cell. Biol., December 15, 2003; 23(24): 9361 - 9374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. McManaman, W. Zabaronick, J. Schaack, and D. J. Orlicky Lipid droplet targeting domains of adipophilin J. Lipid Res., April 1, 2003; 44(4): 668 - 673. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
