AJP - Endo Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab (May 20, 2003). doi:10.1152/ajpendo.00015.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/3/E645    most recent
00015.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suarez, C.
Right arrow Articles by Becu-Villalobos, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suarez, C.
Right arrow Articles by Becu-Villalobos, D.
Submitted on January 9, 2003
Accepted on May 11, 2003

Angiotensin II phosphorylation of extracellular signal regulated kinases in rat anterior pituitary cells

Cecilia Suarez1, Graciela Diaz-Torga1, Arturo Gonzalez-Iglesias1, Jorge Vela1, Alejandro Mladovan1, Alberto Baldi1, and Damasia Becu-Villalobos1*

1 Instituto de Biologia y Medicina Experimental-CONICET, Buenos Aires, Argentina

* To whom correspondence should be addressed. E-mail: dbecu{at}dna.uba.ar.

We studied the effects of Ang II on ERK 1/2 (ERK) phosphorylation in rat pituitary cells. Ang II increased ERK phosphorylation in a time and concentration dependent way. Maximum effect was obtained at 5 min, at a concentration of 10-100 nM. The effect of 100 nM Ang II was blocked by the AT1 antagonist, Dup753, by the PLC inhibitor U73122, and by the MEK antagonist PD98059. Ang II-induced increase in pERK was insensitive to PTX blockade and PKC depletion or inhibition. The effect was also abrogated by chelating intracellular calcium with BAPTA-AM or TMB-8, by depleting intracellular calcium stores with a 30 min pretreatment with EGTA, and by pretreatment with herbimycin A and PP1, two c-Src tyrosine kinase inhibitors. It was attenuated by AG 1478 an inhibitor of EGF R activation. Therefore, in the rat pituitary the increase of pERK is a Gq and PLC dependent process, which involves an increase in intracellular calcium and activation of a c-Src tyrosine kinase, transactivation of the EGF-R and the activation of MEK. Finally, the response of ERK activation by Ang II is altered in hyperplastic pituitary cells, in which calcium mobilization evoked by Ang II is also modified.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Vela, M. I. Perez-Millan, D. Becu-Villalobos, and G. Diaz-Torga
Different kinases regulate activation of voltage-dependent calcium channels by depolarization in GH3 cells
Am J Physiol Cell Physiol, September 1, 2007; 293(3): C951 - C959.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
F. Zhang, Q. Zhang, A. Tengholm, and A. Sjoholm
Involvement of JAK2 and Src kinase tyrosine phosphorylation in human growth hormone-stimulated increases in cytosolic free Ca2+ and insulin secretion
Am J Physiol Cell Physiol, September 1, 2006; 291(3): C466 - C475.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Suarez, G. Diaz-Torga, A. Gonzalez-Iglesias, C. Cristina, and D. Becu-Villalobos
Upregulation of angiotensin II type 2 receptor expression in estrogen-induced pituitary hyperplasia
Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E786 - E794.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.