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Oxidative stress stimulates skeletal muscle glucose uptake through a phosphatidylinositol 3-kinase-dependent pathway

¹Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga; ²Department of Health and Sports Science, Nippon Medical School, Kanagawa, Japan; ³Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts; ⁴Faculty of Education and Human Sciences, University of Yamanashi, Yamanashi; and ⁵Liberal Arts Division, Kisarazu National College of Technology, Chiba, Japan

Submitted 8 March 2007 ; accepted in final form 21 February 2008

	ABSTRACT

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
We determined the acute effects of oxidative stress on glucose uptake and intracellular signaling in skeletal muscle by incubating muscles with reactive oxygen species (ROS). Xanthine oxidase (XO) is a superoxide-generating enzyme that increases ROS. Exposure of isolated rat extensor digitorum longus (EDL) muscles to Hx/XO (Hx/XO) for 20 min resulted in a dose-dependent increase in glucose uptake. To determine whether the mechanism leading to Hx/XO-stimulated glucose uptake is associated with the production of H₂O₂, EDL muscles from rats were preincubated with the H₂O₂ scavenger catalase or the superoxide scavenger superoxide dismutase (SOD) prior to incubation with Hx/XO. Catalase treatment, but not SOD, completely inhibited the increase in Hx/XO-stimulated 2-deoxyglucose (2-DG) uptake, suggesting that H₂O₂ is an intermediary leading to Hx/XO-stimulated glucose uptake with incubation. Direct H₂O₂ also resulted in a dose-dependent increase in 2-DG uptake in isolated EDL muscles, and the maximal increase was threefold over basal levels at a concentration of 600 µmol/l H₂O₂. H₂O₂-stimulated 2-DG uptake was completely inhibited by the phosphatidylinositol 3-kinase (PI3K) inhibitor wortmannin, but not the nitric oxide inhibitor N^G-monomethyl-L-arginine. H₂O₂ stimulated the phosphorylation of Akt Ser⁴⁷³ (7-fold) and Thr³⁰⁸ (2-fold) in isolated EDL muscles. H₂O₂ at 600 µmol/l had no effect on ATP concentrations and did not increase the activities of either the 1 or 2 catalytic isoforms of AMP-activated protein kinase. These results demonstrate that acute exposure of muscle to ROS is a potent stimulator of skeletal muscle glucose uptake and that this occurs through a PI3K-dependent mechanism.

hydrogen peroxide; Akt phosphorylation; adenosine monophosphate-activated protein kinase

There are many other stimuli that can increase glucose transport in skeletal muscle, including, but not limited to, hypoxia (6, 23), hyperosmotic stress (18, 22), 5-aminoimidazole-4-carboxamide 1-β-D-ribonucleoside (AICAR) (23, 42), metformin (57), leptin (3), bradykinin (30), the nitric oxide donor sodium nitroprusside (SNP) (14, 25), and reactive oxygen species (ROS) such as hydrogen peroxide (H₂O₂) (7, 53). Many of these stimuli are widely believed to utilize the same signaling network by which exercise increases glucose transport, and in recent years this has been hypothesized to involve the AMP-activated protein kinase (AMPK). However, it is now clear that AMPK may mediate some but not all of these insulin-independent stimuli, and there are likely to be several distinct or overlapping signaling pathways leading to glucose transport in skeletal muscle (1, 25, 28, 43–45). For example, we have shown that the nitric oxide donor SNP increases skeletal muscle glucose uptake through a mechanism that is distinct from both the insulin- and contraction-signaling pathways (25).

Oxidative stress occurs in a cellular system when the production of free radical moieties exceeds the antioxidant capacity of that system. To date, two biochemical pathways have been identified as potential sources of intracellular free radical generation in skeletal muscle. Mitochondria continually produce free radicals in response to conditions, such as exercise, that increase oxygen uptake, via a reaction involving complexes I and III of the respiratory chain (8). Another source for free radicals is generation by xanthine oxidase (XO) (41), a ubiquitously expressed enzyme that is present in skeletal muscle (24). XO produces free radicals in the presence of oxygen and hypoxanthine (Hx) or XO, ATP degradation products that can be generated during skeletal muscle contraction (24). H₂O₂ can be generated from this reaction, and H₂O₂ has been shown to increase glucose transport under some experimental conditions (7). Thus, although H₂O₂ may be an important positive regulator of glucose transport in skeletal muscle, little is known about the signaling mechanisms that may mediate this process.

In the current study, we determined the effects of acute exposure of skeletal muscle to pharmacological agents that produce ROS and then assessed potential signaling mechanisms that might mediate the effects of these agents on glucose transport. We first used exogenous Hx/XO exposure of isolated skeletal muscle, a model system that has been proposed to reproduce what occurs in vivo where XO has been found in the blood vessel walls. We found that Hx/XO resulted in a dose-dependent increase in 2-deoxyglucose (2-DG) uptake in isolated EDL muscles, likely due to the generation of H₂O₂ in vitro. Direct incubation of muscles with H₂O₂ also resulted in dose-dependent increase in glucose transport, which occurred via an AMPK-independent, but PI3K-dependent signaling mechanism. Thus, ROS can positively mediate glucose transport in skeletal muscle.

	RESEARCH DESIGN AND METHODS

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
Experimental animals. Male Sprague-Dawley rats weighing 40 g were purchased from Kyudo (Tosu, Japan) and Taconic Farms (Germantown, MA). Animals were housed in an animal room maintained at 23°C with a 12:12-h light-dark cycle and fed standard laboratory chow and water ad libitum. All protocols for animal use and euthanasia were reviewed and approved by the Institutional Animal Care and Use Committees of Saga Medical School and the Joslin Diabetes Center.

Materials. XO, H₂O₂, wortmannin, catalase, superoxide dismutase (SOD), D-glucose, and pyruvic acid were purchased from Sigma Chemical (St. Louis, MO). Hx was purchased from Wako Chemical (Osaka, Japan). N^G-nitro-L-arginine monomethyl ester (L-NMMA) was purchased from Calbiochem (San Diego, CA). 2-Deoxy-D-[1,2-³H]glucose and D-[¹⁴C]mannitol were purchased from PerkinElmer (Boston, MA).

Muscle incubations. Rats were fed ad libitum until 8:00 AM on the day of the experiment, and the experiment commenced at 1:00 PM. Animals were killed by decapitation, and the EDL muscles were rapidly dissected. Both ends of each muscle were tied with suture (4-0 silk) and mounted on an incubation apparatus. The muscles were preincubated in 5 ml of Krebs-Ringer bicarbonate buffer (KRB; 117 mM NaCl, 4.7 mM KCl, 2.5 mM CaCl₂, 1.2 mM KH₂PO₄, 1.2 mM MgSO₄, and 24.6 mM NaHCO₃, pH 7.5) containing 2 mmol/l pyruvate at 37°C for 30 min in the presence or absence of insulin (50 mU/ml) or AICAR (2 mM), and then incubated in the presence or absence of XO (0.2–200 mU/ml) with Hx (1 mmol/l) or H₂O₂ (0.03–24 mmol/l) at 37°C for 20 min. When added, the inhibitors catalase (3,000 mU/ml), SOD (300 U/ml), wortmannin (100 nmol/l), or L-NMMA (100 nmol/l) were present throughout the entire incubation and present at least 30 min prior to stimulation. The buffers were continuously gassed with 95% O₂-5% CO₂. Muscles were then immediately frozen in liquid nitrogen and used for the measurement of 2-DG uptake or analyzed for muscle enzymes and metabolites. For the measurement of H₂O₂ in the incubation buffer in the presence or absence of XO with Hx, the BIOXYTECH H₂O₂-560 assay was used (Portland, OR).

2-DG uptake. 2-DG uptake was measured in 2 ml of KRB containing 1 mmol/l 2-deoxy-D-[1,2-³H]glucose (1.5 µCi/ml) and 7 mmol/l D-[¹⁴C]mannitol (0.45 µCi/ml) at 30°C for 10 min. Muscles were processed, radioactivity was determined by liquid scintillation counting for dual labels, and 2-DG uptake was calculated as previously described (5). Isolated EDL muscles from rat were used for the current experiments. Mice were not used for these studies because the effects of H₂O₂ exposure on glucose uptake under our incubation conditions were not observed in mouse EDL muscles. The lack of stimulation by H₂O₂ is similar to other findings in our laboratory where stimuli that increase glucose uptake in the rat (e.g., metformin), have no effect in the mouse (N. Fujii and L. J. Goodyear, unpublished observation). We suspect this may be due to the much higher level of spontaneous activity of mice compared with rats or humans, activating the glucose transport system in the basal state.

Assays for muscle enzymes and metabolites. To measure ATP, ADP, and AMP concentrations, frozen muscles were weighed and then homogenized using a polytron (Kinematica, Switzerland) in trichloroacetic acid at –10°C, and centrifuged at 14,000 g for 10 min at 4°C. The supernatant of the homogenates was neutralized with 0.5 N tri-n-octylamine and then centrifuged at 14,000 g at 4°C. The supernatant was passed through a membrane filter with a 0.45 µm pore size and the sample was then analyzed by high-performance liquid chromatography (HPLC) as described previously (51).

For the measurement of isoform-specific AMPK activity, muscles were homogenized in ice-cold lysis buffer (1:20 wt/vol) containing 20 mmol/l Tris·HCl (pH 7.4), 1% Triton X-100, 50 mmol/l NaCl, 250 mmol/l sucrose, 50 mmol/l NaF, 5 mmol/l sodium pyrophosphate, 2 mmol/l DTT, 4 mg/l leupeptin, 50 mg/l trypsin inhibitor, 0.1 mmol/l benzamidine and 0.5 mmol/l PMSF and centrifuged at 14,000 g for 20 min at 4°C. The supernatants (200 µg protein) were immunoprecipitated with isoform-specific antibodies to the 1 or 2 catalytic subunits of AMPK and protein A/G beads. These are anti-peptide antibodies made to the amino acid sequences DFYLATSPPDSFLDDHHLTR (339–358) of 1 and MDDSAMHIPPGLKPH (352–366) of 2 and protein A/G beads. Immnoprecipitates were washed twice in lysis buffer and twice in wash buffer (240 mmol/l HEPES and 480 mmol/l NaCl). Kinase reactions were performed in 40 mmol/l HEPES (pH 7.0), 0.2 mmol/l SAMS peptide (synthetic substrate for AMPK), 0.2 mmol/l AMP, 80 mmol/l NaCl, 0.8 mmol/l DTT, 5 mmol/l MgCl₂, and 0.2 mmol/l ATP (2 µCi [-³²P]ATP) in a final volume of 40 µl for 20 min at 30°C (13). At the end of the reaction, a 20-µl aliquot was removed and spotted on Whatman P81 paper. The papers were washed six times in 1% phosphoric acid and once with acetone. ³²P incorporation was quantitated with a scintillation counter, and kinase activity was expressed as fold increases compared with basal samples.

Immunoblotting for AMPK Thr¹⁷² phosphorylation. Equal amounts of muscle lysates (40 µg protein) prepared as described above were separated by SDS-PAGE, transferred to a PVDF membrane, and blocked in TBS-T containing 5% nonfat milk for 1 h at room temperature. The membranes were incubated overnight at 4°C with phospho-AMPK (Thr¹⁷²) antibody (Upstate Biotechnology). Bound primary antibodies were detected with anti-rabbit or anti-mouse immunoglobulin-horseradish-peroxidase-linked whole antibody. The membranes were washed with TBS-T and then incubated with enhanced chemiluminescence reagents and exposed to film. Bands were visualized and quantified using ImageQuant software (Molecular Dynamics).

Immunoblotting for Akt Ser⁴⁷³ and Thr³⁰⁸ phosphorylation. Pulverized muscle was homogenized with a Polytron in an ice-cold buffer (20 mM Tris, pH 7.5, 5 mM EDTA, 10 mM Na₃PO₄, 100 mMNaF, 2 mM Na₃VO₄, 1% Nonidet P-40, 10 µM leupeptin, 3 mM benzamidine, 10 µg/ml aprotinin, and 1 mM phenylmethylsulfonyl fluoride). Homogenates were rotated end over end for 1 h at 4°C and then centrifuged at 14,000 g for 10 min at 4°C. The supernatants were collected and stored at –80°C until analyzed.

Akt Ser⁴⁷³ and Thr³⁰⁸ phosphorylation was assessed by immunoblotting. An equal amount of muscle protein (80 µg) was separated by SDS-PAGE, transferred to a PVDF membrane, and blocked in TBS-T containing 5% nonfat milk for 1 h at room temperature. The membranes were incubated with an antibody specific for Akt phosphorylated on Ser⁴⁷³ (1:1,000 dilution) or specific for Akt phosphorylated on Thr³⁰⁸ (1:2,000 dilution; Cell Signaling Technology) overnight at 4°C. Membranes were incubated with horseradish peroxidase-conjugated secondary antibody (1:2,000) in TBS-T containing 5% nonfat milk for 1 h at room temperature, and antibody binding was visualized as described above.

Statistical analysis. Data are expressed as means ± SE. For comparison of two means, an unpaired Student's t-test was performed. The effect of H₂O₂ on insulin or AICAR-stimulated 2-DG uptake was compared by a one-way analysis of variance (ANOVA) with Fisher's protected least significant difference-test.

	RESULTS

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Effects of Hx/XO on glucose uptake in isolated EDL muscles. To determine whether ROS stimulates muscle glucose uptake, isolated EDL muscles were first incubated in buffer containing Hx in the presence of XO (Hx/XO). Incubation of EDL muscles with Hx/XO for 20 min resulted in a dose-dependent increase in 2-DG uptake (Fig. 1A). The maximal increase was 2.4-fold over basal levels at a concentration of 40 mU/ml XO in the presence of 1 mM Hx and did not increase further with 200 mU/ml XO. XO or Hx alone did not affect 2-DG uptake in isolated EDL muscle (data not shown), suggesting that reaction product(s) that appears in response to exposure of the combination of Hx and XO leads to increased muscle glucose uptake.

View larger version (8K): [in this window] [in a new window]   Fig. 1. Hypoxanthine/xanthine oxidase (Hx/XO)-stimulated 2-deoxyglucose (2-DG) uptake (A) and effects of catalase or superoxide dismutase (SOD) on Hx/XO-stimulated 2-DG uptake (B) in isolated extensor digitorum longus (EDL) muscles. A: isolated EDL muscles were stimulated in the absence or presence of 0.2–200 mU/ml XO with 1 mM Hx for 20 min followed by measurement of 2-DG uptake, as described in RESEARCH AND METHODS; n = 5–7 per group. B: isolated EDL muscles were preincubated in KRB buffer containing 3,000 U/ml catalase or 300 U/ml SOD for 30 min and then stimulated in 40 mU/ml XO with 1 mM Hx for 20 min followed by the measurement of 2-DG uptake. Data are means ± SE; n = 5 per group. *P < 0.05 vs. basal condition.

We next determined the effects of direct H₂O₂ incubation on skeletal muscle glucose uptake. H₂O₂ exposure for 20 min resulted in a dose-dependent increase in 2-DG uptake in isolated EDL muscles (Fig. 2). The maximal increase was threefold over basal levels at H₂O₂ concentrations of 600 µmol/l and 1,200 µmol/l. With concentrations greater than 2.0 mmol/l there was a reduction in glucose uptake, and by 24 mmol/l H₂O₂ glucose uptake was at basal levels. These findings suggest that there is an optimal concentration of H₂O₂ for the stimulation of glucose uptake and that at higher concentrations H₂O₂ is either ineffective or inhibitory.

View larger version (8K): [in this window] [in a new window]   Fig. 2. Effects of H2O2 (0.1–24 mM) on 2-DG uptake in isolated EDL muscles. Isolated EDL muscles were stimulated in the absence or presence of 0.1–24 mM H2O2 for 20 min followed by the measurement of 2-DG uptake, as described in RESEARCH AND METHODS. Data are means ± SE; n = 5–7 per group.

Effects of H₂O₂ on AICAR-stimulated glucose uptake and AMPK activity. There are multiple mechanisms that increase glucose uptake in skeletal muscle, and in recent years AMPK has been suggested to be a key signaling protein regulating insulin-independent glucose uptake. To address the hypothesis that H₂O₂ stimulates glucose uptake through activation of AMPK, we carried out several experiments assessing glucose uptake and AMPK activity. First, we determined whether the combination of a maximally stimulating dose of H₂O₂ plus maximal AICAR had additive effects on glucose uptake. Isolated EDL muscles were incubated in KRB buffer in the absence or presence of 2 mmol/l AICAR for 50 min. H₂O₂ (600 µmol/l) was added during last 20 min of the 50-min incubation period. The combination of H₂O₂ plus AICAR had partially additive effects on skeletal muscle glucose uptake (Fig. 3), suggesting different mechanisms for H₂O₂- and AICAR-stimulated glucose uptake in skeletal muscle. There was also a partially additive effect of the combination Hx/XO plus AICAR on skeletal muscle glucose uptake (data not shown). Consistent with this finding, incubation of isolated EDL muscles with H₂O₂ (600 µmol/l) for a total of 50 min had no effect on either AMPK1 or AMPK2 activities assessed using an immune complex assay (Fig. 4A). Phosphorylation of AMPK on Thr¹⁷², a key regulatory site for AMPK activity, was also unchanged with H₂O₂ incubation for 5, 10, or 20 min (Fig. 4B). H₂O₂ incubation did not enhance AICAR-stimulated AMPK phosphorylation (data not shown). Finally, H₂O₂ had no effect on the muscle metabolites ATP, ADP, and AMP (Table 1). Collectively, these data suggest that AMPK is not involved as an intermediary in the signaling cascade leading to H₂O₂-stimulated glucose uptake in skeletal muscle.

View larger version (11K): [in this window] [in a new window]   Fig. 3. Effects of H2O2 on 5-aminoimidazole-4-carboxamide 1-β-D-ribonucleoside (AICAR)-stimulated 2-DG uptake in isolated EDL muscles. Isolated EDL muscles were incubated in KRB buffer in the absence or presence of 2 mmol/l AICAR for 50 min. H2O2 incubation was at a concentration (600 µmol/l) that elicited maximal increase in glucose uptake, as indicated in Fig. 2, and was added during the last 20 min the of 50-min incubation period. Data are means ± SE; n = 6–8 per group.

View larger version (21K): [in this window] [in a new window]   Fig. 4. Effects of H2O2 on AMPK1 and -2 activity (A) and effects of H2O2 on phosphorylation level of AMPK (Thr172) (B) in isolated EDL muscles. Isolated EDL muscles were incubated in KRB buffer in the presence of H2O2 at a concentration (600 µmol/l) that elicited maximal increase in glucose uptake, as indicated in Fig. 2. Data are means ± SE; n = 6 per group.

View larger version (14K): [in this window] [in a new window]   Fig. 5. Effects of wortmannin on H2O2-stimulated 2-DG uptake (A) and effects of H2O2 on Akt Ser473 and Thr308 phosphorylation (B) in isolated EDL muscles. A: isolated EDL muscles were preincubated in KRB buffer in the presence or absence wortmannin (100 nM) followed by treatment with H2O2 at a concentration (600 µmol/l) that elicited maximal increase in H2O2-stimulated glucose uptake, as indicated in Fig. 2. Since wortmannin was dissolved in DMSO, which may act as a scavenger of superoxide anion, 2-DG uptake was measured in the presence of 0.2% DMSO for another control; n = 5–6 per group. B: isolated EDL muscles were incubated in KRB buffer in the presence or absence of H2O2 (600 µmol/l); n = 6 per group. Data are means ± SE. **P < 0.01 vs. control.

View larger version (11K): [in this window] [in a new window]   Fig. 6. Effects of H2O2 on insulin-stimulated 2-DG uptake in isolated EDL muscles. Isolated EDL muscles were preincubated in KRB buffer in the presence or absence of insulin at a concentration (300 nM) that induced maximal increase in insulin-stimulated glucose uptake. H2O2 incubation was at a concentration (600 µmol/l) that elicited maximal increase in glucose uptake, as indicated in Fig. 2, and was added during last 20 min of the 50-min incubation period. Data are means ± SE; n = 7–8 per group.

View larger version (12K): [in this window] [in a new window]   Fig. 7. Effects of NG-monomethyl-L-arginine (L-NMMA) on H2O2-stimulated 2-DG uptake in isolated EDL muscles. Isolated EDL muscles were preincubated in KRB buffer in the presence or absence of L-NMMA (100 nmol/l) for 30 min, followed with treatment with H2O2 at a concentration (600 µmol/l) that induces maximal increase in H2O2-stimulated glucose uptake, as indicated in Fig. 2. Data are means ± SE; n = 5–6 per group.

	DISCUSSION

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

Despite the finding nearly 15 years ago that H₂O₂ can stimulate glucose transport in isolated rat epitrochlearis muscles (7), in recent years there has been little focus on elucidating cell signaling mechanisms that may mediate the metabolic effects of oxidative stress. There are distinct signaling cascades that stimulate glucose transport in muscle, including insulin-dependent signaling, exercise or muscle contraction, AMPK, and nitric oxide-mediated signaling. In the present study, H₂O₂-stimulated 2-DG uptake in isolated EDL muscles was completely inhibited in the presence of the PI3K inhibitor wortmannin. This finding is similar to that of another study that found that the treatment of 3T3-L1 adipocytes with wortmannin completely blocks the subsequent activation of Akt phosphorylation in response to cell stimulation with exogenous H₂O₂ (38). Since wortmannin could theoretically act through nonspecific inhibition of a PI3K-independent signaling mechanism(11), we assessed Akt phosphorylation, a downstream kinase in PI3K signaling. Our findings that Akt Ser⁴⁷³ and Thr³⁰⁸ phosphorylation was significantly increased in muscle in the presence of H₂O₂ at a concentration that increased glucose transport, reinforces the concept that H₂O₂-stimulated 2-DG uptake involves PI3K-Akt signaling in skeletal muscles.

The mechanism by which H₂O₂ increases Akt phosphorylation in our system is not clear. One possibility is that protein tyrosine phosphates (PTPases) are inactivated in our system, as has been shown in other cell types (2, 37, 39). PTP1B, in particular, is considered to be a major candidate for the negative regulation of insulin action, functioning to decrease insulin receptor and IRS-1 tyrosine phosphorylation. Although we cannot rule out a role for PTPB1 in H₂O₂-stimulated PI3K/Akt signaling because we did not measure phosphatase activity, we think this is unlikely, because we did not observe any effect of H₂O₂ on IRS-1 Tyr⁶¹² phosphorylation (data not shown). Thus, the mechanism by which H₂O₂ increases PI3K/Akt signaling will be an interesting area for future investigation.

Exogenous oxidants such as H₂O₂ have been known to be capable of directly activating the insulin receptor and consequently downstream signaling targets in liver (20) and adipocytes (12, 29, 35). However, the effects of oxidative stress on insulin-stimulated glucose transport and signaling have been inconsistent, raising questions as to whether oxidative stress is a positive or negative regulator of insulin signaling and glucose transport. The effect of H₂O₂ on insulin signaling is complex and could be dependent on the sequence in which cells or tissues are exposed to H₂O₂ or insulin. If administered prior to insulin, micromolar concentrations of H₂O₂ exposure to cultured cells can inhibit insulin action (4, 21, 27). In L6 muscle cells, insulin-stimulated glucose transport was suppressed when muscle cells were incubated with insulin and H₂O₂ (4) or with insulin following the prior stimulation of H₂O₂-producing glucose oxidase (27), suggesting that H₂O₂ is likely to downregulate insulin signaling. Consistent with this hypothesis, in NIH-B cells, H₂O₂ potently inhibited insulin signaling and resulted in the development of insulin resistance (21). On the other hand, mice overexpressing glutathione peroxidase-1, an intracellular selenoprotein that reduces H₂O₂, developed insulin resistance (40), suggesting that H₂O₂ is a positive mediator of insulin action. If H₂O₂ is given on its own or following insulin, it can be an insulin mimetic or partly additive to insulin (7), and in the current study, we found that H₂O₂ stimulates glucose transport in isolated muscles and that the combination with insulin and H₂O₂ had partially additive effects on muscle glucose transport. These results suggest that H₂O₂ does not impair maximal insulin-stimulated glucose transport and that skeletal muscle plays a role in glucose handling in response to H₂O₂ stimulation.

A recent study showed that ROS have a causal role in different forms of insulin resistance (26). In vivo, this becomes even more complex when physical exercise, stress, disease state, and circulating systemic factors are all interacting. The incidence of insulin resistance and diabetes is inversely correlated with tissue antioxidant enzyme activities and blood antioxidant concentrations (15, 17, 54). In contrast, exercise generates nitric oxide and ROS and has a key role in the prevention of diabetes and improved insulin action in skeletal muscle. One explanation of these conflicting results is that excessive and/or chronic oxidative stress would induce insulin resistance in various cell types, whereas optimal and acute oxidative stress can have positive effects on glucose transport. In future studies, it will be important to determine the different mechanisms by which acute exposure of oxidative stress caused by exercise and chronic exposure of oxidative stress caused by hyperglycemia alters glucose homeostasis.

Nitric oxide and ROS exert complex effects on muscle contraction, metabolism, and gene expression (47). We previously reported that SNP, a nitric oxide donor, enhances glucose uptake in skeletal muscle and is associated with activation of the 1 catalytic subunit of AMPK (25). AMPK is a key regulatory enzyme in the control of cell-energy homoeostasis, acting as a "fuel gauge" and functioning to increase ATP generation under conditions of increased energy expenditure (22, 46). Recent studies report increased AMPK activity in response to oxidative stress in NIH-3T3 cells (9) and in isolated rat epitrochlearis (53) and EDL muscles (50). AMPK1 activity increased in a time- and dose-dependent manner in H₂O₂-stimulated epitrochlearis muscles, whereas AMPK2 activity was unaffected (53). The activation of AMPK1 was blocked in the presence of the antioxidant N-acetyl-L-cysteine (NAC), whereas NAC only partially (50%) decreased H₂O₂-stimulated glucose uptake (53). Sandstrom et al. (50) also showed that NAC reduced contraction-stimulated glucose uptake as well as AMPK activity and phosphorylation in EDL by 50%, but NAC did not alter insulin-, hypoxia-, or AICAR-stimulated glucose uptake. These findings suggest that endogenous H₂O₂ production may play an important role in contraction-stimulated glucose uptake in rat skeletal muscle that may partially be mediated by AMPK activation. In contrast to these studies, we found no effect of H₂O₂ on either AMPK1 or -2 activities in isolated EDL muscles. Furthermore, we show no effect of H₂O₂ on AMPK Thr¹⁷² phosphorylation.

The lack of AMPK activation with H₂O₂ is also consistent with our data showing that H₂O₂ did not significantly alter ATP concentrations and the AMP/ATP ratio in the incubated muscles. A recent study demonstrated that addition of pyruvate to the perfusate prevented the H₂O₂-mediated reduction in cardiac mechanical dysfunction, activation of myocardial AMPK activity, increase in AMPK phosphorylation, and the increase in the Cr/PCr ratio (34). This may suggest that an effect of H₂O₂ on lowering energy levels in heart muscle was prevented by addition of pyruvate to the buffer. When we measured the dose response curve for H₂O₂-stimulated 2-DG uptake in isolated EDL muscles, we observed that higher doses (>2 mM) of H₂O₂ induced a dose-dependent decrease in 2-DG uptake and an increase in muscle stiffness. Since the dose (0.6 mM) of H₂O₂ in our experiment was much lower than that in previous studies (3 mM) (50, 53), the control of cell-energy homoeostasis might have been better regulated in our muscles. Therefore, the difference compared with previous studies in terms of AMPK activity and ATP levels in H₂O₂-stimulated muscles may be due to a different dose of H₂O₂. Our finding that the combination of H₂O₂ plus AICAR had additive effects on skeletal muscle glucose uptake suggests that the H₂O₂ doses of 0.6 mM do not stimulate glucose transport through an AMPK-dependent mechanism. Our finding that the NOS inhibitor L-NMMA had no effect on H₂O₂-stimulated glucose uptake in isolated skeletal muscles demonstrates that the nitric oxide signaling mechanism is also not functioning to increase glucose transport with H₂O₂.

In summary, the current study demonstrates that Hx/XO-stimulated glucose uptake in isolated EDL muscles is associated with the production of H₂O₂ and that H₂O₂ stimulates muscle glucose transport. The mechanism by which H₂O₂ increases glucose uptake does not involve AMPK or nitric oxide signaling. Instead, PI3K is necessary for H₂O₂-stimulated glucose transport in skeletal muscle. Interestingly, this signaling mechanism is likely to be only partially overlapping with insulin signaling to glucose uptake. In contrast to the hypothesis that there are two signaling pathways to glucose uptake in skeletal muscle, it is now apparent that there are multiple mechanisms, some distinct and some overlapping. Elucidating these pathways will be important for understanding optimal treatments for muscle in insulin resistance.

	ACKNOWLEDGMENTS

 
This work was supported by KAKENHI 13780027 and 16500412 (Y. Higaki), Saga Medical School Grants (Y. Higaki), and National Institutes of Health Grants AR-45670 and AR-42238 (L. J. Goodyear).

	FOOTNOTES

 

Address for reprint requests and other correspondence: Y. Higaki, Dept. of Preventive Medicine, Faculty of Medicine, Saga University, Saga 849-8501, Japan (e-mail: higaki{at}cc.saga-u.ac.jp)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 

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