|
Table 2.
Metabolite concentrations and CK isoenzyme activities in hearts from
rats fed either control diet or diet supplemented with creatine
or  -GPA for 6 wk
|
|
| Feeding Group |
Creatinefree,
nmol/mg
protein |
PCr,
nmol/mg
protein |
Creatinetotal,
nmol/mg
protein |
-GPAtotal,
nmol/mg
protein |
ATP,
nmol/mg
protein |
CK activity,
IU/mg
protein |
Mito-CK
(%) |
MM-CK
(%) |
MB-CK
(%) |
BB-CK
(%)
|
|
| Control |
41.5 ± 5.0 |
35.2 ± 3.6 |
76.7 ± 5.3 |
ND |
31.7 ± 4.1 |
6.1 ± 0.6 |
28.1 ± 3.3 |
49.7 ± 6.8 |
18.6 ± 3.3 |
3.5 ± 1.3 |
| 3%
Creatine |
46.0 ± 2.9 |
39.6 ± 1.7 |
85.6 ± 2.1* |
ND |
31.6 ± 0.8 |
6.2 ± 0.7 |
24.6 ± 0.7 |
53.8 ± 3.3 |
18.5 ± 1.3 |
3.1 ± 0.3 |
| 1.5%
-GPA |
ND |
ND |
15.3 ± 2.7* |
72.9 ± 5.7* |
30.9 ± 1.7 |
6.2 ± 0.9 |
25.3 ± 0.1 |
48.1 ± 2.4 |
21.5 ± 2.1 |
5.0 ± 0.2 |
|
|
Data are means ± SD for 4 rats/group. CK, creatine kinase;
PCr, phosphocreatine; mito, mitochondrial; MM, MB, and BB,
muscle-muscle, muscle-brain, and brain-brain, respectively; ND, not
determined.
*
P < 0.01 vs. control.
|
|
