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This Article Full Text (PDF) All Versions of this Article: 297/6/E1349    most recent ajpendo.00505.2009v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Jorgensen, W. Articles by Quistorff, B. PubMed PubMed Citation Articles by Jorgensen, W. Articles by Quistorff, B.

RESEARCH ARTICLE

Changed mitochondrial function by pre-and/or post partum diet alterations in sheep

¹University of Copenhagen ²Rigshospitalet, section 9312 ³Copenhagen Muscle Research Centre ⁴University of Copenhagen - Department of Basic Animal and Veterinary Sciences - Animal Nutrition

Submitted 18 August 2009 ; revised 30 September 2009 ; accepted in final form 5 October 2009

In a sheep model we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 month of life. We examined mitochondrial function in permeabilized muscle fibres from the lambs at 6 months of age (adolescence) and after 24 months of age (adulthood). The post-partum H-diet to the lambs induced a ~30% increase (P<0.05) of mitochondrial VO_2-max and a ~50% increase (P<0.05) of the respiratory coupling ratio (RCR), combined with lower levels of UCP3 and PGC-1 mRNA levels (P<0.05). These effects proved to be reversible by a normal diet from 6 to 24 months of age. However, at 24 months a long term effect, of the maternal gestational diet restriction (fetal programming) became evident as a lower VO_2-max (~40%, P<0.05), a lower state-4 respiration (~40%, P<0.05), and lower RCR (~15%, P<0.05). Both PGC-1 and UCP3 mRNA levels were increased (P<0.05). Two analysed muscles were differently affected, and muscle rich in type I fibres was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced VO_2-max in adulthood, results from gestational under-nutrition. Post natal high-fat diet results in a pronounced RCR and VO_2-max increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood.

Metabolic syndrome; high-fat diet; nutrient restriction; maternal diet