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This Article Full Text Full Text (PDF) All Versions of this Article: 297/3/E717    most recent 00282.2009v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Jung, S.-R. Articles by Sweet, I. R. PubMed PubMed Citation Articles by Jung, S.-R. Articles by Sweet, I. R.

A highly energetic process couples calcium influx through L-type calcium channels to insulin secretion in pancreatic β-cells

Department of Medicine, Diabetes and Obesity Center of Excellence, University of Washington at South Lake Union, Seattle, Washington

Submitted 30 April 2009 ; accepted in final form 2 July 2009

Calcium (Ca²⁺) influx is required for the sustained secretion of insulin and is accompanied by a large rate of energy usage. We hypothesize that the energy usage reflects a process [Ca²⁺/metabolic coupling process (CMCP)] that couples Ca²⁺ to insulin secretion by pancreatic islets. The aim of the study was to test this hypothesis by testing the effect of inhibiting candidate Ca²⁺-sensitive proteins proposed to play a critical role in the CMCP. The effects of the inhibitors on oxygen consumption rate (OCR), a reflection of ATP usage, and insulin secretion rate (ISR) were compared with those seen when L-type Ca²⁺ channels were blocked with nimodipine. We reasoned that if a downstream Ca²⁺-regulated site was responsible for the OCR associated with the CMCP, then its inhibition should mimic the effect of nimodipine. Consistent with previous findings, nimodipine decreased glucose-stimulated OCR by 36% and cytosolic Ca²⁺ by 46% and completely suppressed ISR in rat pancreatic islets. Inhibitors of three calmodulin-sensitive proteins (myosin light-chain kinase, calcineurin, and Ca²⁺/calmodulin-dependent protein kinase II) did not meet the criteria. In contrast, KN-62 severed the connection between Ca²⁺ influx, OCR, and ISR without interfering with Ca²⁺ influx. In the presence of nimodipine or KN-62, potentiators of ISR, acetylcholine, GLP-1, and arginine had little effect on insulin secretion, suggesting that the CMCP is also essential for the amplification of ISR. In conclusion, a KN-62-sensitive process directly mediates the effects of Ca²⁺ influx via L-type Ca²⁺ channels on OCR and ISR, supporting the essential role of the CMCP in mediating ISR.

oxygen consumption; calmodulin; islet; KN-62