AJP - Endo Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 296: E1262-E1268, 2009. First published February 24, 2009; doi:10.1152/ajpendo.90511.2008
0193-1849/09 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
296/6/E1262    most recent
90511.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Madani, R.
Right arrow Articles by Mohamed-Ali, V.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Madani, R.
Right arrow Articles by Mohamed-Ali, V.

RANTES release by human adipose tissue in vivo and evidence for depot-specific differences

Rana Madani,1,2,* Kalypso Karastergiou,1,4,* Nicola C. Ogston,1 Nazar Miheisi,1 Rahul Bhome,1 Nora Haloob,1 Garry D. Tan,3 Fredrik Karpe,3 James Malone-Lee,2 Majid Hashemi,2 Marjan Jahangiri,4 and Vidya Mohamed-Ali1

1Centre for Clinical Pharmacology, Division of Medicine, University College London and 2Whittington Hospital, Archway Campus, University College London, London; 3Oxford Centre for Diabetes, Endocrinology, and Metabolism, Churchill Hospital, University of Oxford, Oxford; and 4Department of Cardiac and Vascular Sciences, St George's University of London, London, United Kingdom

Submitted 17 June 2008 ; accepted in final form 10 February 2009

Obesity is associated with elevated inflammatory signals from various adipose tissue depots. This study aimed to evaluate release of regulated on activation, normal T cell expressed and secreted (RANTES) by human adipose tissue in vivo and ex vivo, in reference to monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) release. Arteriovenous differences of RANTES, MCP-1, and IL-6 were studied in vivo across the abdominal subcutaneous adipose tissue in healthy Caucasian subjects with a wide range of adiposity. Systemic levels and ex vivo RANTES release were studied in abdominal subcutaneous, gastric fat pad, and omental adipose tissue from morbidly obese bariatric surgery patients and in thoracic subcutaneous and epicardial adipose tissue from cardiac surgery patients without coronary artery disease. Arteriovenous studies confirmed in vivo RANTES and IL-6 release in adipose tissue of lean and obese subjects and release of MCP-1 in obesity. However, in vivo release of MCP-1 and RANTES, but not IL-6, was lower than circulating levels. Ex vivo release of RANTES was greater from the gastric fat pad compared with omental (P = 0.01) and subcutaneous (P = 0.001) tissue. Epicardial adipose tissue released less RANTES than thoracic subcutaneous adipose tissue in lean (P = 0.04) but not obese subjects. Indexes of obesity correlated with epicardial RANTES but not with systemic RANTES or its release from other depots. In conclusion, RANTES is released by human subcutaneous adipose tissue in vivo and in varying amounts by other depots ex vivo. While it appears unlikely that the adipose organ contributes significantly to circulating levels, local implications of this chemokine deserve further investigation.

C-C ligand 5; monocyte chemoattractant protein-1; interleukin-6; arteriovenous differences


Address for reprint requests and other correspondence: V. Mohamed-Ali, Adipokines and Metabolism Research Group, Centre for Clinical Pharmacology, Div. of Medicine, University College London, 5 University St., London, UK WC1 6JJ (e-mail: rmhavma{at}ucl.ac.uk)






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2009 by the American Physiological Society.