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Am J Physiol Endocrinol Metab 296: E1059-E1066, 2009. First published March 3, 2009; doi:10.1152/ajpendo.90609.2008
0193-1849/09 $8.00
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Estradiol upregulates the expression of oxytocin receptor in colon in rats

Mei Feng,1,2 Junfang Qin,1,2 Chao Wang,1,2 Yanfang Ye,1,2 Shuanglian Wang,1,2 Dongping Xie,1,2 Paulus S. Wang,3 and Chuanyong Liu1,2

1Department of Physiology, Shandong University School of Medicine; 2Key Laboratory of Medical Neuroiology of Shandong University, Jinan, China; and 3Department of Physiology, School of Medicine, National Yang-Ming University Taiwan, Taipei, Taiwan

Submitted 18 July 2008 ; accepted in final form 23 February 2009

The study was designed to investigate the effect of estradiol on the excitatory effect of oxytocin (OT) on colon motility. Female Wistar rats were used, and some of them were ovariectomized (OVX) and treated with vehicle or estradiol (E2). A plastic balloon made of condom was inserted into colon to monitor the change of colonic pressure in vivo. Longitudinal muscle strips of distal colon were prepared to monitor the spontaneous contraction of colon in vitro. Expression of OT receptor (OTR) was investigated by Western blot analysis. Expression of OTR mRNA was detected by RT-PCR. Immunohistochemistry was used to locate OTR. In OVX rats, pretreatment of E2 (4–100 µg/kg sc) dose-dependently increased the excitatory effect of OT on colon motility both in vivo and in vitro and increased the expression of OTR and OTR mRNA in colon. Systemic administration of OT excited the colon motility in vivo in rats at perioda of proestrus and estrus but did not influence it at diestrus period, when the concentration of plasma E2 was lowest in the estrous cycle. Pretreatment of atosiban, the specific OTR antagonist, and TTX, the blocker of voltage-dependent sodium channel on nerve fiber, attenuated the excitatory effect of OT on colon motility. OTR was located in myenteric plexus of colon. These results suggested that E2 increased the excitatory effect of OT on colon motility by upregulating the expression of OTR in myenteric plexus.

colon; motility


Address for reprint requests and other correspondence: C. Liu, Dept. of Physiology, Medical School of Shandong University, Jinan 250012, China (e-mail: liucy{at}sdu.edu.cn)






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