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Am J Physiol Endocrinol Metab 295: E1369-E1379, 2008. First published October 14, 2008; doi:10.1152/ajpendo.90555.2008
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25-Hydroxycholesterol-3-sulfate regulates macrophage lipid metabolism via the LXR/SREBP-1 signaling pathway

Yongjie Ma,1 Leyuan Xu,1 Daniel Rodriguez-Agudo,1 Xiaobo Li,1 Douglas M. Heuman,1 Phillip B. Hylemon,2 William M. Pandak,1 and Shunlin Ren1

1Departments of Medicine and 2Microbiology/Immunology, Virginia Commonwealth University/McGuire Veterans Affairs Medical Center, Richmond, Virginia

Submitted 1 July 2008 ; accepted in final form 9 October 2008

The oxysterol receptor LXR is a key transcriptional regulator of lipid metabolism. LXR increases expression of SREBP-1, which in turn regulates at least 32 genes involved in lipid synthesis and transport. We recently identified 25-hydroxycholesterol-3-sulfate (25HC3S) as an important regulatory molecule in the liver. We have now studied the effects of 25HC3S and its precursor, 25-hydroxycholesterol (25HC), on lipid metabolism as mediated by the LXR/SREBP-1 signaling in macrophages. Addition of 25HC3S to human THP-1-derived macrophages markedly decreased nuclear LXR protein levels. 25HC3S administration was followed by dose- and time-dependent decreases in SREBP-1 mature protein and mRNA levels. 25HC3S decreased the expression of SREBP-1-responsive genes, acetyl-CoA carboxylase-1, and fatty acid synthase (FAS) as well as HMGR and LDLR, which are key proteins involved in lipid metabolism. Subsequently, 25HC3S decreased intracellular lipids and increased cell proliferation. In contrast to 25HC3S, 25HC acted as an LXR ligand, increasing ABCA1, ABCG1, SREBP-1, and FAS mRNA levels. In the presence of 25HC3S, 25HC, and LXR agonist T0901317, stimulation of LXR targeting gene expression was repressed. We conclude that 25HC3S acts in macrophages as a cholesterol satiety signal, downregulating cholesterol and fatty acid synthetic pathways via inhibition of LXR/SREBP signaling. A possible role of oxysterol sulfation is proposed.

25-hydroxycholesterol; sulfated 25-hydroxycholesterol; cholesterol and triglyceride metabolism; oxysterol sulfation; oxysterol metabolism


Address for reprint requests and other correspondence: S. Ren, Veterans Affairs McGuire Medical Center/Virginia Commonwealth University, Research 151, 1201 Broad Rock Blvd., Richmond, VA 23249 (e-mail: shunlin.ren{at}va.gov)




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