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Am J Physiol Endocrinol Metab 295: E938-E946, 2008. First published August 19, 2008; doi:10.1152/ajpendo.90462.2008
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Corticotropin-releasing hormone stimulates SGK-1 kinase expression in cultured hippocampal neurons via CRH-R1

Hui Sheng,1,4,* Tingting Sun,1,4,* Binhai Cong,1,4 Ping He,1,4 Yanmin Zhang,1,4 Jin Yan,3 Changlin Lu,2,4 and Xin Ni1,4

Departments of 1Physiology, 2Neurobiology, and 3Psychology, Second Military Medical University; and 4Key Laboratory of Molecular Neurobiology, Ministry of Education, Shanghai, China

Submitted 23 May 2008 ; accepted in final form 12 August 2008

Corticotropin-releasing hormone (CRH) has been shown to exhibit various functions in hippocampus. In the present study, we examined the effect of CRH on the expression of serum/glucocorticoid-inducible protein kinase-1 (SGK-1), a novel protein kinase, in primary cultured hippocampal neurons. A dose-dependent increase in mRNA and protein levels of SGK-1 as well as frequency of SGK-1-positive neurons occurred upon exposure to CRH (1 pmol/l to 10 nmol/l). These effects can be reversed by the specific CRH-R1 antagonist antalarmin but not by the CRH-R2 antagonist astressin 2B. Blocking adenylate cyclase (AC) activity with SQ22536 and PKA with H89 completely prevented CRH-induced mRNA and protein expression of SGK-1. Blockage of PLC or PKC did not block CRH-induced SGK-1 expression. Our results suggest that CRH act on CRH-R1 to stimulate SGK-1 mRNA and protein expression in cultured hippocampal neurons via a mechanism that is involved in AC/PKA signaling pathways.

serum/glucocorticoid-inducible protein kinase-1; corticotropin-releasing hormone receptor-1; adenylate cyclase; adenosine 3'5'-cyclic monophosphate-dependent protein kinase; phospholipase C; protein kinase C


Address for reprint requests and other correspondence: X. Ni, Dept. of Physiology, Second Military Medical University, 800 Xiangyin Rd., Shanghai 200433, P. R. China (e-mail: nixin{at}smmu.edu.cn)






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