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This Article Full Text Full Text (PDF) All Versions of this Article: 293/5/E1153    most recent 00186.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zhou, H. R. Articles by Claycombe, K. J. Search for Related Content PubMed PubMed Citation Articles by Zhou, H. R. Articles by Claycombe, K. J.

Obesity-associated mouse adipose stem cell secretion of monocyte chemotactic protein-1

Departments of ¹Food Science and Human Nutrition and ²Neurology and Ophthalmology, Michigan State University, East Lansing, Michigan

Submitted 20 April 2007 ; accepted in final form 11 August 2007

Studies showed that monocyte chemotactic protein-1 (MCP-1) concentrations are increased in obesity. In our current study, we demonstrate that plasma MCP-1 level in leptin-deficient ob/ob mice is significantly higher than in lean mice. Furthermore, we determined that basal adipose tissue MCP-1 mRNA levels are significantly higher in ob/ob mice compared with lean mice. To determine the mechanisms underlying obesity-associated increases in plasma and adipose tissue MCP-1 levels, we determined adipose tissue cell type sources of MCP-1 production. Our data show that adipose tissue stem cells (CD34⁺), macrophages (F4/80⁺), and stromal vascular fraction (SVF) cells express significantly higher levels of MCP-1 compared with adipocytes under both basal and lipopolysaccharide (LPS)-stimulated conditions. Furthermore, basal and LPS-induced MCP-1 secretion levels were the same for both adipose F4/80⁺ and CD34⁺ cells, whereas adipose CD34⁺ cells have twofold higher cell numbers (30% of total SVF cells) compared with F4/80⁺ macrophages (15%). Our data also show that CD34⁺ cells from visceral adipose tissue depots secrete significantly higher levels of MCP-1 ex vivo when compared with CD34⁺ cells from subcutaneous adipose tissue depots. Taken together, our data suggest that adipose CD34⁺ stem cells may play an important role in obesity-associated increases in plasma MCP-1 levels.

monocyte chemotactic protein-1; adipose stem cell; adipose tissue; obesity