AJP - Endo Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Am J Physiol Endocrinol Metab 293: E1062-E1068, 2007. First published July 31, 2007; doi:10.1152/ajpendo.00045.2007
0193-1849/07 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
293/4/E1062    most recent
00045.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lira, V. A.
Right arrow Articles by Criswell, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lira, V. A.
Right arrow Articles by Criswell, D. S.

Nitric oxide increases GLUT4 expression and regulates AMPK signaling in skeletal muscle

Vitor A. Lira, Quinlyn A. Soltow, Jodi H. D. Long, Jenna L. Betters, Jeff E. Sellman, and David S. Criswell

Department of Applied Physiology and Kinesiology, Center for Exercise Science, University of Florida, Gainesville, Florida

Submitted 17 January 2007 ; accepted in final form 29 July 2007

Nitric oxide (NO) and 5'-AMP-activated protein kinase (AMPK) are involved in glucose transport and mitochondrial biogenesis in skeletal muscle. Here, we examined whether NO regulates the expression of the major glucose transporter in muscle (GLUT4) and whether it influences AMPK-induced upregulation of GLUT4. At low levels, the NO donor S-nitroso-N-penicillamine (SNAP, 1 and 10 µM) significantly increased GLUT4 mRNA (~3-fold; P < 0.05) in L6 myotubes, and cotreatment with the AMPK inhibitor compound C ablated this effect. The cGMP analog 8-bromo-cGMP (8-Br-cGMP, 2 mM) increased GLUT4 mRNA by ~50% (P < 0.05). GLUT4 protein expression was elevated 40% by 2 days treatment with 8-Br-cGMP, whereas 6 days treatment with 10 µM SNAP increased GLUT4 expression by 65%. Cotreatment of cultures with the guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one prevented the SNAP-induced increase in GLUT4 protein. SNAP (10 µM) also induced significant phosphorylation of {alpha}-AMPK and acetyl-CoA carboxylase and translocation of phosphorylated {alpha}-AMPK to the nucleus. Furthermore, L6 myotubes exposed to 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) for 16 h presented an approximately ninefold increase in GLUT4 mRNA, whereas cotreatment with the non-isoform-specific NOS inhibitor NG-nitro-L-arginine methyl ester, prevented ~70% of this effect. In vivo, GLUT4 mRNA was increased 1.8-fold in the rat plantaris muscle 12 h after AICAR injection, and this induction was reduced by ~50% in animals cotreated with the neuronal and inducible nitric oxide synthases selective inhibitor 1-(2-trifluoromethyl-phenyl)-imidazole. We conclude that, in skeletal muscle, NO increases GLUT4 expression via a cGMP- and AMPK-dependent mechanism. The data are consistent with a role for NO in the regulation of AMPK, possibly via control of cellular activity of AMPK kinases and/or AMPK phosphatases.

5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside; guanosine 3',5'-cyclic monophosphate; L6 myotubes; compound C; NG-nitro-L-arginine methyl ester; glucose transporter 4; adenosine 5'-monophosphate-activated protein kinase



Address for reprint requests and other correspondence: D. Criswell, P.O. Box 118206, Center for Exercise Science, Univ. of Florida, Gainesville, FL 32611 (e-mail: dcriswell{at}hhp.ufl.edu)




This article has been cited by other articles:


Home page
J. Biol. Chem.Home page
J. R. Gayen, M. Saberi, S. Schenk, N. Biswas, S. M. Vaingankar, W. W. Cheung, S. M. Najjar, D. T. O'Connor, G. Bandyopadhyay, and S. K. Mahata
A Novel Pathway of Insulin Sensitivity in Chromogranin A Null Mice: A CRUCIAL ROLE FOR PANCREASTATIN IN GLUCOSE HOMEOSTASIS
J. Biol. Chem., October 16, 2009; 284(42): 28498 - 28509.
[Abstract] [Full Text] [PDF]


Home page
Hum Mol GenetHome page
M. Wehling-Henricks, M. Oltmann, C. Rinaldi, K. H. Myung, and J. G. Tidball
Loss of positive allosteric interactions between neuronal nitric oxide synthase and phosphofructokinase contributes to defects in glycolysis and increased fatigability in muscular dystrophy
Hum. Mol. Genet., September 15, 2009; 18(18): 3439 - 3451.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
J. F. Ndisang, N. Lane, and A. Jadhav
Upregulation of the heme oxygenase system ameliorates postprandial and fasting hyperglycemia in type 2 diabetes
Am J Physiol Endocrinol Metab, May 1, 2009; 296(5): E1029 - E1041.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
M. Foller, M. Sopjani, S. Koka, S. Gu, H. Mahmud, K. Wang, E. Floride, E. Schleicher, E. Schulz, T. Munzel, et al.
Regulation of erythrocyte survival by AMP-activated protein kinase
FASEB J, April 1, 2009; 23(4): 1072 - 1080.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
J. F. Ndisang and A. Jadhav
Heme oxygenase system enhances insulin sensitivity and glucose metabolism in streptozotocin-induced diabetes
Am J Physiol Endocrinol Metab, April 1, 2009; 296(4): E829 - E841.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
B. A. Murphy, K. A. Fakira, Z. Song, A. Beuve, and V. H. Routh
AMP-activated protein kinase and nitric oxide regulate the glucose sensitivity of ventromedial hypothalamic glucose-inhibited neurons
Am J Physiol Cell Physiol, January 1, 2009; 297(3): C750 - C758.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
E. Karnieli and M. Armoni
Transcriptional regulation of the insulin-responsive glucose transporter GLUT4 gene: from physiology to pathology
Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E38 - E45.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
J. A. Drenning, V. A. Lira, C. G. Simmons, Q. A. Soltow, J. E. Sellman, and D. S. Criswell
Nitric oxide facilitates NFAT-dependent transcription in mouse myotubes
Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1088 - C1095.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. J. Green, T. A. Duhamel, G. P. Holloway, J. W. Moule, D. W. Ranney, A. R. Tupling, and J. Ouyang
Rapid upregulation of GLUT-4 and MCT-4 expression during 16 h of heavy intermittent cycle exercise
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R594 - R600.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.