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This Article Full Text Full Text (PDF) All Versions of this Article: 292/6/E1917    most recent 00654.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Williams, K. B. Articles by DeLuca, H. F. Search for Related Content PubMed PubMed Citation Articles by Williams, K. B. Articles by DeLuca, H. F.

Characterization of intestinal phosphate absorption using a novel in vivo method

Department of Biochemistry and Department of Nutritional Sciences, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin

Submitted 30 November 2006 ; accepted in final form 27 January 2007

A new, completely in vivo method of measuring the rate of intestinal phosphate absorption has been developed. As expected from previous in vitro and ex vivo measurements, intestinal phosphate absorption is potently and rapidly stimulated by 1,25-dihydroxyvitamin D₃. The response is saturated with as little as 11.3 ng of 1,25-dihydroxyvitamin D₃ per day, consistent with a genomic mechanism. The effect of 1,25-dihydroxyvitamin D₃ disappears when the dosing solution of phosphate is at 2 M, suggesting that 1,25-dihydroxyvitamin D₃ stimulates active transport of phosphate but not diffusion of phosphate. Finally, unlike findings resulting from in vitro or ex vivo experiments, no evidence in vivo was obtained that phosphate absorption requires sodium or is inhibited by potassium.

vitamin D; phosphorus; intestine

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