HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/5/E947    most recent 00128.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lomenick, J. P. Articles by Handwerger, S. Search for Related Content PubMed PubMed Citation Articles by Lomenick, J. P. Articles by Handwerger, S.

Transcription factor FOXF1 regulates growth hormone variant gene expression

Department of Pediatrics, University of Cincinnati College of Medicine, and Division of Endocrinology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio

Submitted 21 March 2006 ; accepted in final form 7 June 2006

Deletion analysis of the human growth hormone variant (GHV) promoter in transient transfection studies in BeWo choriocarcinoma and HepG2 cells indicated that the region extending from nt –158/+57 retained full transcriptional activity. DNase I footprint analysis of the fragment revealed a protected region at nt –82/–77, which is in a putative FOXF1/FOXF2 binding site. Supershift assays using an antiserum to human FOXF1 demonstrated that the protected region binds FOXF1. Overexpression of FOXF1 in BeWo and HepG2 cells induced the GHV promoter, whereas overexpression of FOXF2 was without effect. Mutagenesis of the FOXF1/FOXF2 site reduced basal promoter activity by 50–60% and markedly attenuated transactivation of the promoter by FOXF1. These studies indicate that FOXF1 induces GHV expression by interaction with a FOXF1/FOXF2 cis-element in the proximal promoter.

forkhead box F1; placenta; transcription; promoter activity