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Differential expression of uterine calcium transporter 1 and plasma membrane Ca²⁺ ATPase 1b during rat estrous cycle

¹Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea; ²Department of Obstetrics and Gynecology, British Columbia Children's and Women's Hospital, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada; and ³Xenotransplantation Research Center, Seoul National University, Seoul, Republic of Korea

Submitted 9 September 2005 ; accepted in final form 8 February 2006

Calcium-related proteins include the calcium transporters 1 and 2 (CaT1 and CaT2), plasma membrane Ca²⁺-ATPase 1b (PMCA1b), and calbindin-D9k and -D28k. The expression of CaT1 and PMCA1b and their potential roles in the uterine tissue remain to be clarified. Thus, in the present study, the expression patterns of CaT1 and PMCA1b were examined to predict their roles in rat uterus during the estrous cycle. Both CaT1 and PMCA1b mRNAs were detected in rat uterus. Uterine CaT1 mRNA was highly expressed at diestrus compared with proestrus, whereas PMCA1b expression was not altered during the estrus cycle. To evaluate the sex steroids involved in uterine CaT1 mRNA regulation, 17-estradiol (E₂) and/or progesterone (P₄) were injected into immature rats. Treatment with P₄ or E₂ plus P₄ resulted in an increase in CaT1 mRNA, but a synergetic effect of E₂ plus P₄ was not detected. Uterine CaT1 mRNA was induced by P₄ in a time- and dose-dependent manner, with maximal transcript detected 12 h after the final P₄ injection. Treatment with RU486, a progesterone receptor (PR) antagonist, completely blocked P₄-induced CaT1 mRNA, indicating that P₄ regulates CaT1 mRNA expression via a PR-mediated pathway. In addition, CaT1 mRNA was expressed in uterine endometrium and glandular endometrium at diestrus in P₄-treated rats. Together, these results suggest that CaT1 is regulated by P₄ at diestrus via a PR-dependent pathway.

transient receptor potential vanilloid 6; uterus

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