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This Article Full Text Full Text (PDF) All Versions of this Article: 289/1/E145    most recent 00563.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Monjo, M. Articles by Roca, P. Search for Related Content PubMed PubMed Citation Articles by Monjo, M. Articles by Roca, P.

₂- to ₃-Adrenoceptor switch in 3T3-L1 preadipocytes and adipocytes: modulation by testosterone, 17-estradiol, and progesterone

Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d'Investigació en Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain

Submitted 29 November 2004 ; accepted in final form 9 February 2005

Sex steroid hormones are important factors in the determination of fat distribution and accumulation. The aim of this study was to investigate the effect of testosterone (T), 17-estradiol (17E), and progesterone (P) on adrenergic receptor (AR) gene expression in 3T3-L1 preadipocytes and adipocytes and their relation to the proliferation and differentiation processes. Our data clearly show that _2A-AR was the highest AR subtype expressed in preadipocytes, whereas in mature adipocytes was by far ₃-AR. In the differentiation process to adipocytes, _2A-AR expression was decreased to 0.3-fold (P < 0.01), whereas ₃-AR was upregulated 578-fold (P < 0.001) compared with preadipocytes. In addition, the expression of _2A-AR in preadipocytes was increased upon incubation with T, 17E, and P, and a stimulation of proliferation was also observed in 17E- and P-treated cells. In mature adipocytes, 17E and P enhanced both _2A- and ₃-AR gene expression (although the effects on ₃-AR mRNA levels could be more relevant, since ₃-AR was the most highly expressed), whereas T only increased _2A-AR mRNA levels. Leptin and adipocyte fatty acid-binding protein mRNA levels were higher after 17E and P treatment, possibly indicating a proadipogenic effect of these hormones. In conclusion, this study indicates that AR gene expression is affected by these hormones in both preadipocytes and adipocytes, which could have potential importance when considering the role of ARs in the mechanisms underlying the sex-related differences in adipose tissue regional distribution.

sex steroid hormones; _2A-; ₁-; ₂-; and ₃-adrenergic receptors; cell proliferation; 3T3-L1 adipocyte

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