HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/6/E1128    most recent 00003.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Miyazawa-Hoshimoto, S. Articles by Saito, Y. Search for Related Content PubMed PubMed Citation Articles by Miyazawa-Hoshimoto, S. Articles by Saito, Y.

Roles of degree of fat deposition and its localization on VEGF expression in adipocytes

Departments of ¹Clinical Cell Biology, ²Genome Research and Clinical Application, and ³Division of Applied Translational Research, Graduate School of Medicine, Chiba University, Chiba, Japan

Submitted 5 January 2004 ; accepted in final form 17 December 2004

Vascular endothelial growth factor (VEGF) is an important angiogenic factor and is expressed in wide variety of cell types. In this study, we investigated the mechanism of VEGF production in adipocytes in three sets of experiments. First, to clarify the relation between plasma VEGF concentrations and their expressions in adipose tissues, we investigated the genetically obese db/db and KK-A^y mice. Plasma VEGF concentrations in obese mice were significantly higher than in control and were related to adiposity. VEGF expressions in visceral fat were enhanced during growth and were related to fat deposition. Next, to demonstrate the relation between VEGF production and lipid accumulation in adipocytes, we analyzed VEGF mRNA expression and its protein secretion in 3T3-L1 cells. VEGF production was enhanced during lipid accumulation in 3T3-L1 cells after adipocyte conversion. Next, to clarify the role of anatomic localization on VEGF expression in adipocytes, we implanted 3T3-L1 cells into visceral or subcutaneous fat in athymic mice. 3T3-L1 cells implanted into the mesenteric area expressed more VEGF mRNA than that into the subcutaneous area. Plasma VEGF concentration in the mice implanted in visceral fat was higher than in controls. These results suggest that both the anatomic localization and the lipid accumulation are important for the VEGF production in adipocytes.

vascular endothelial growth factor; fat distribution; cytokine; gene expression; 3T3-L1 cells

This article has been cited by other articles:

				S. Ledoux, I. Queguiner, S. Msika, S. Calderari, P. Rufat, J.-M. Gasc, P. Corvol, and E. Larger Angiogenesis Associated With Visceral and Subcutaneous Adipose Tissue in Severe Human Obesity Diabetes, December 1, 2008; 57(12): 3247 - 3257. [Abstract] [Full Text] [PDF]

				T. Kawamura, K. Murakami, H. Bujo, H. Unoki, M. Jiang, T. Nakayama, and Y. Saito Matrix Metalloproteinase-3 Enhances the Free Fatty Acids-Induced VEGF Expression in Adipocytes Through Toll-Like Receptor 2 Experimental Biology and Medicine, October 1, 2008; 233(10): 1213 - 1221. [Abstract] [Full Text] [PDF]

				A. J. Glassford, P. Yue, A. Y. Sheikh, H. J. Chun, S. Zarafshar, D. A. Chan, G. M. Reaven, T. Quertermous, and P. S. Tsao HIF-1 regulates hypoxia- and insulin-induced expression of apelin in adipocytes Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1590 - E1596. [Abstract] [Full Text] [PDF]

				G. Rega, C. Kaun, S. Demyanets, S. Pfaffenberger, K. Rychli, P.J. Hohensinner, S.P. Kastl, W.S. Speidl, T.W. Weiss, J.M. Breuss, et al. Vascular Endothelial Growth Factor Is Induced by the Inflammatory Cytokines Interleukin-6 and Oncostatin M in Human Adipose Tissue In Vitro and in Murine Adipose Tissue In Vivo Arterioscler. Thromb. Vasc. Biol., July 1, 2007; 27(7): 1587 - 1595. [Abstract] [Full Text] [PDF]

				S. Nishimura, I. Manabe, M. Nagasaki, Y. Hosoya, H. Yamashita, H. Fujita, M. Ohsugi, K. Tobe, T. Kadowaki, R. Nagai, et al. Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels Diabetes, June 1, 2007; 56(6): 1517 - 1526. [Abstract] [Full Text] [PDF]