AJP - Endo Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 287: E616-E621, 2004. First published May 18, 2004; doi:10.1152/ajpendo.00150.2004
0193-1849/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
287/4/E616    most recent
00150.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (45)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bruce, C. R.
Right arrow Articles by Dyck, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bruce, C. R.
Right arrow Articles by Dyck, D. J.

Cytokine regulation of skeletal muscle fatty acid metabolism: effect of interleukin-6 and tumor necrosis factor-{alpha}

Clinton R. Bruce and David J. Dyck

Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Submitted 1 April 2004 ; accepted in final form 14 May 2004

IL-6 and TNF-{alpha} have been associated with insulin resistance and type 2 diabetes. Furthermore, abnormalities in muscle fatty acid (FA) metabolism are strongly associated with the development of insulin resistance. However, few studies have directly examined the effects of either IL-6 or TNF-{alpha} on skeletal muscle FA metabolism. Here, we used a pulse-chase technique to determine the effect of IL-6 (50–5,000 pg/ml) and TNF-{alpha} (50–5,000 pg/ml) on FA metabolism in isolated rat soleus muscle. IL-6 (5,000 pg/ml) increased exogenous and endogenous FA oxidation by ~50% (P < 0.05) but had no effect on FA uptake or incorporation of FA into endogenous lipid pools. In contrast, TNF-{alpha} had no effect on FA oxidation but increased FA incorporation into diacylglycerol (DAG) by 45% (P < 0.05). When both IL-6 (5,000 pg/ml) and insulin (10 mU/ml) were present, IL-6 attenuated insulin's suppressive effect on FA oxidation, increasing exogenous FA oxidation (+37%, P < 0.05). Furthermore, in the presence of insulin, IL-6 reduced the esterification of FA to triacylglycerol by 22% (P < 0.05). When added in combination with IL-6 or leptin (10 µg/ml), the TNF-{alpha}-induced increase in DAG synthesis was inhibited. In conclusion, the results demonstrate that IL-6 plays an important role in regulating fat metabolism in muscle, increasing rates of FA oxidation, and attenuating insulin's lipogenic effects. In contrast, TNF-{alpha} had no effect on FA oxidation but increased FA incorporation into DAG, which may be involved in the development of TNF-{alpha}-induced insulin resistance in skeletal muscle.

triacylglycerol; diacylglycerol; fatty acid oxidation; insulin resistance


Address for reprint requests and other correspondence: Clinton R. Bruce, Dept. of Human Biology and Nutritional Sciences, Univ. of Guelph, Guelph, ON, Canada N1G 2W1 (E-mail: crbruce{at}uoguelph.ca)




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
B. K. Pedersen
Edward F. Adolph Distinguished Lecture: Muscle as an endocrine organ: IL-6 and other myokines
J Appl Physiol, October 1, 2009; 107(4): 1006 - 1014.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
X. Yang, Y. Zhou, R. Jin, and C. Chan
Reconstruct modular phenotype-specific gene networks by knowledge-driven matrix factorization
Bioinformatics, September 1, 2009; 25(17): 2236 - 2243.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Liesa, M. Palacin, and A. Zorzano
Mitochondrial Dynamics in Mammalian Health and Disease
Physiol Rev, July 1, 2009; 89(3): 799 - 845.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. R. Steinberg and B. E. Kemp
AMPK in Health and Disease
Physiol Rev, July 1, 2009; 89(3): 1025 - 1078.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. R. Bruce, A. J. Hoy, N. Turner, M. J. Watt, T. L. Allen, K. Carpenter, G. J. Cooney, M. A. Febbraio, and E. W. Kraegen
Overexpression of Carnitine Palmitoyltransferase-1 in Skeletal Muscle Is Sufficient to Enhance Fatty Acid Oxidation and Improve High-Fat Diet-Induced Insulin Resistance
Diabetes, March 1, 2009; 58(3): 550 - 558.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
B. K. Pedersen and M. A. Febbraio
Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6
Physiol Rev, October 1, 2008; 88(4): 1379 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
A. G Holmes, J. L Mesa, B. A Neill, J. Chung, A. L Carey, G. R Steinberg, B. E Kemp, R. J Southgate, G. I Lancaster, C. R Bruce, et al.
Prolonged interleukin-6 administration enhances glucose tolerance and increases skeletal muscle PPAR{alpha} and UCP2 expression in rats
J. Endocrinol., August 1, 2008; 198(2): 367 - 374.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Weigert, M. Dufer, P. Simon, E. Debre, H. Runge, K. Brodbeck, H. U. Haring, and E. D. Schleicher
Upregulation of IL-6 mRNA by IL-6 in skeletal muscle cells: role of IL-6 mRNA stabilization and Ca2+-dependent mechanisms
Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1139 - C1147.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. L. Mullen, A. C. Smith, K. A. Junkin, and D. J. Dyck
Globular adiponectin resistance develops independently of impaired insulin-stimulated glucose transport in soleus muscle from high-fat-fed rats
Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E83 - E90.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. K. Pedersen, M. A. Febbraio, and R. A. Mooney
Interleukin-6 does/does not have a beneficial role in insulin sensitivity and glucose homeostasis
J Appl Physiol, February 1, 2007; 102(2): 814 - 816.
[Full Text] [PDF]

Home page
 DiabetesHome page
A. L. Carey, G. R. Steinberg, S. L. Macaulay, W. G. Thomas, A. G. Holmes, G. Ramm, O. Prelovsek, C. Hohnen-Behrens, M. J. Watt, D. E. James, et al.
Interleukin-6 Increases Insulin-Stimulated Glucose Disposal in Humans and Glucose Uptake and Fatty Acid Oxidation In Vitro via AMP-Activated Protein Kinase.
Diabetes, October 1, 2006; 55(10): 2688 - 2697.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. G. Smith and G. E. O. Muscat
Orphan nuclear receptors: therapeutic opportunities in skeletal muscle
Am J Physiol Cell Physiol, August 1, 2006; 291(2): C203 - C217.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. A. Frost, G. J. Nystrom, and C. H. Lang
Multiple Toll-like receptor ligands induce an IL-6 transcriptional response in skeletal myocytes
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2006; 290(3): R773 - R784.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
H. Bruunsgaard
Physical activity and modulation of systemic low-level inflammation
J. Leukoc. Biol., October 1, 2005; 78(4): 819 - 835.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. Bach, D. Naon, S. Pich, F. X. Soriano, N. Vega, J. Rieusset, M. Laville, C. Guillet, Y. Boirie, H. Wallberg-Henriksson, et al.
Expression of Mfn2, the Charcot-Marie-Tooth Neuropathy Type 2A Gene, in Human Skeletal Muscle: Effects of Type 2 Diabetes, Obesity, Weight Loss, and the Regulatory Role of Tumor Necrosis Factor {alpha} and Interleukin-6
Diabetes, September 1, 2005; 54(9): 2685 - 2693.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. R. Berggren, M. W. Hulver, and J. A. Houmard
Fat as an endocrine organ: influence of exercise
J Appl Physiol, August 1, 2005; 99(2): 757 - 764.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Weigert, A. M. Hennige, K. Brodbeck, H. U. Haring, and E. D. Schleicher
Interleukin-6 acts as insulin sensitizer on glycogen synthesis in human skeletal muscle cells by phosphorylation of Ser473 of Akt
Am J Physiol Endocrinol Metab, August 1, 2005; 289(2): E251 - E257.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. M. W. Petersen and B. K. Pedersen
The anti-inflammatory effect of exercise
J Appl Physiol, April 1, 2005; 98(4): 1154 - 1162.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
Highlights from the Literature
Physiology, October 1, 2004; 19(5): 233 - 239.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.