| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Department of Surgical Science and Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden; and 2Department of Kinesiology, University of Wisconsin-Madison, Madison, Wisconsin 53706
Submitted 6 April 2004 ; accepted in final form 12 May 2004
Exposing isolated rat skeletal muscle to 5-aminoimidazole-4-carboxamide-1-
-D-ribofuranoside [AICAR, a pharmacological activator of AMP-activated protein kinase (AMPK)] plus serum leads to a subsequent increase in insulin-stimulated glucose transport (Fisher JS, Gao J, Han DH, Holloszy JO, and Nolte LA. Am J Physiol Endocrinol Metab 282: E18–E23, 2002). Our goal was to determine whether preincubation of primary human skeletal muscle cells with human serum and AICAR (Serum+AICAR) would also induce a subsequent elevation in insulin-stimulated glucose uptake. Cells were preincubated for 1 h under 4 conditions: 1) without AICAR or serum (Control), 2) with serum, 3) with AICAR, or 4) with Serum+AICAR. Some cells were then collected for immunoblot analysis to assess phosphorylation of AMPK (pAMPK) and its substrate acetyl-CoA carboxylase (ACC). Other cells were incubated for an additional 4 h without AICAR or serum and then used to measure basal or insulin-stimulated 2-deoxyglucose (2-DG) uptake. Level of pAMPK was increased (P < 0.01) for myotubes exposed to Serum+AICAR vs. all other groups. Phosphorylated ACC (pACC) levels were higher for both Serum+AICAR (P < 0.05) and AICAR (P < 0.05) vs. Control and Serum groups. Basal (P < 0.05) and 1.2 nM insulin-stimulated (P < 0.005) 2-DG uptake was higher for Serum vs. all other preincubation conditions at equal insulin concentration. Regardless of insulin concentration (0, 1.2, or 18 nM), 2-DG was unaltered in cells preincubated with Serum+AICAR vs. Control cells. In contrast to results with isolated rat skeletal muscle, increasing the pAMPK and pACC in human myocytes via preincubation with Serum+AICAR was insufficient to lead to a subsequent enhancement in insulin-stimulated glucose uptake.
exercise; skeletal muscle; glucose transport; 5-aminoimidazole-4-carboxamide-1--D-ribofuranoside; AMP-activated protein kinase
This article has been cited by other articles:
|
|
 |
|
  N. Fujii, N. Jessen, and L. J. Goodyear AMP-activated protein kinase and the regulation of glucose transport Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E867 - E877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Christopher, C. Rantzau, Z.-P. Chen, R. Snow, B. Kemp, and F. P. Alford Impact of in vivo fatty acid oxidation blockade on glucose turnover and muscle glucose metabolism during low-dose AICAR infusion Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E1131 - E1140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Camacho, D. B. Lacy, F. D. James, E. P. Donahue, and D. H. Wasserman 5-Aminoimidazole-4-carboxamide-1-{beta}-D-ribofuranoside renders glucose output by the liver of the dog insensitive to a pharmacological increment in insulin Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1039 - E1043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Smith, P. B. Patil, and J. S. Fisher AICAR and hyperosmotic stress increase insulin-stimulated glucose transport J Appl Physiol, September 1, 2005; 99(3): 877 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Pencek, J. Shearer, R. C. Camacho, F. D. James, D. B. Lacy, P. T. Fueger, E. P. Donahue, W. Snead, and D. H. Wasserman 5-Aminoimidazole-4-Carboxamide-1-{beta}-D-Ribofuranoside Causes Acute Hepatic Insulin Resistance In Vivo Diabetes, February 1, 2005; 54(2): 355 - 360. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
