HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Chami, O. Articles by O'Neill, C. Search for Related Content PubMed PubMed Citation Articles by Chami, O. Articles by O'Neill, C.

Components of a platelet-activating factor-signaling loop are assembled in the ovine endometrium late in the estrous cycle

¹Human Reproduction Unit, Department of Physiology, University of Sydney, Royal North Shore Hospital of Sydney, St. Leonards, New South Wales 2065; and ²Faculty of Veterinary Science, University of Sydney, Sydney, New South Wales 2006, Australia

Submitted 11 July 2003 ; accepted in final form 10 February 2004

Pulsatile release of uterine prostaglandin F₂ (PGF₂) induces luteolysis in ruminants. Exogenous PAF is well known to cause PGF₂ release from the ovine uterus. This study examines whether the components of a PAF-signaling loop exist in sheep at the time luteolysis is normally initiated. Day 14 of the cycle was the first day the uterus responded to an infusion of PAF, inducing a significant short-term increase in circulating levels of the PGF₂ metabolite. There was a significant increase of PAF concentration (P < 0.001) in the endometrium and PAF release by tissue explants (P < 0.001) from day 10 to day 16 of the cycle. Endometrial tissue PAF receptor mRNA expression was induced (P < 0.01) by estradiol and progesterone treatment of animals, and transcripts were present between days 10 and 16 of the estrous cycle. Western analysis of endometrial tissue showed marked upregulation of PAF receptor protein expression from day 14 of the cycle, and immunolocalization studies showed that the receptor expression was predominantly around the endometrial glands. PAF:acetylhydrolase was primarily located within the lumen of the endometrial glands. The study shows that a PAF-signaling loop was assembled within the ovine endometrium at the time that PGF₂ pulsatility was first observed.

endometrium; luteolysis; platelet-activating factor; prostaglandin F₂; PAF:acetylhydrolase (EC 3.1.1.47)

This article has been cited by other articles:

				C. O'Neill The role of paf in embryo physiology Hum. Reprod. Update, May 1, 2005; 11(3): 215 - 228. [Abstract] [Full Text] [PDF]