HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Koonen, D. P. Y. Articles by Bonen, A. Search for Related Content PubMed PubMed Citation Articles by Koonen, D. P. Y. Articles by Bonen, A.

Different mechanisms can alter fatty acid transport when muscle contractile activity is chronically altered

¹Department of Molecular Genetics, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, NL-6200 MD Maastricht, The Netherlands; ²Department of Kinesiology, University of Waterloo, Waterloo, Ontario N2L 3G1; ³Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada; ⁴Thrombosis Research LaboratoryOtsuka America Pharmaceutical Inc., Rockville, Maryland 20850; and ⁵Section of Endocrinology and Metabolism, Wake Forest University School of Medicine & Baptist Medical Center, Winston-Salem, North Carolina 27156

Submitted 20 November 2003 ; accepted in final form 2 February 2004

We examined whether skeletal muscle transport rates of long-chain fatty acids (LCFAs) were altered when muscle activity was eliminated (denervation) or increased (chronic stimulation). After 7 days of chronically stimulating the hindlimb muscles of female Sprague-Dawley rats, the LCFA transporter proteins fatty acid translocase (FAT)/CD36 (+43%) and plasma membrane-associated fatty acid-binding protein (FABPpm; +30%) were increased (P < 0.05), which resulted in the increased plasmalemmal content of these proteins (FAT/CD36, +42%; FABPpm +13%, P < 0.05) and a concomitant increase in the LCFA transport rate into giant sarcolemmal vesicles (+44%, P < 0.05). Although the total muscle contents of FAT/CD36 and FABPpm were not altered (P > 0.05) after 7 days of denervation, the LCFA transport rate was markedly decreased (–39%). This was associated with reductions in plasmalemmal FAT/CD36 (–24%) and FABPpm (–28%; P < 0.05). These data suggest that these LCFA transporters were resequestered to their intracellular depot(s) within the muscle. Combining the results from these experiments indicated that changes in rates of LCFA transport were correlated with concomitant changes in plasmalemmal FAT/CD36 and FABPpm, but not necessarily with their total muscle content. Thus chronic alterations in muscle activity can alter the rates of LCFA transport via different mechanisms, either 1) by increasing the total muscle content of FAT/CD36 and FABPpm, resulting in a concomitant increase at the sarcolemma, or 2) by reducing the plasma membrane content of these proteins in the absence of any changes in their total muscle content.

giant vesicles; tibialis anterior; gastrocnemius; denervation; chronic stimulation

This article has been cited by other articles:

				G. P. Holloway, J. Lally, J. G. Nickerson, H. Alkhateeb, L. A. Snook, G. J. F. Heigenhauser, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, L. L. Spriet, et al. Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle J. Physiol., July 1, 2007; 582(1): 393 - 405. [Abstract] [Full Text] [PDF]

				A. Bonen, X.-X. Han, D. D. J. Habets, M. Febbraio, J. F. C. Glatz, and J. J. F. P. Luiken A null mutation in skeletal muscle FAT/CD36 reveals its essential role in insulin- and AICAR-stimulated fatty acid metabolism Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1740 - E1749. [Abstract] [Full Text] [PDF]

				G. P. Holloway, A. B. Thrush, G. J. F. Heigenhauser, N. N. Tandon, D. J. Dyck, A. Bonen, and L. L. Spriet Skeletal muscle mitochondrial FAT/CD36 content and palmitate oxidation are not decreased in obese women Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1782 - E1789. [Abstract] [Full Text] [PDF]

				A. Bonen, A. Chabowski, J. J. F. P Luiken, and J. F. C. Glatz Mechanisms and Regulation of Protein-Mediated Cellular Fatty Acid Uptake: Molecular, Biochemical, and Physiological Evidence Physiology, February 1, 2007; 22(1): 15 - 28. [Full Text] [PDF]

				C. R. Benton, D. P. Y. Koonen, J. Calles-Escandon, N. N. Tandon, J. F. C. Glatz, J. J. F. P. Luiken, J. J. Heikkila, and A. Bonen Differential effects of contraction and PPAR agonists on the expression of fatty acid transporters in rat skeletal muscle J. Physiol., May 15, 2006; 573(1): 199 - 210. [Abstract] [Full Text] [PDF]

				C. Roepstorff, B. Vistisen, K. Roepstorff, and B. Kiens Regulation of plasma long-chain fatty acid oxidation in relation to uptake in human skeletal muscle during exercise Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E696 - E705. [Abstract] [Full Text] [PDF]