AJP - Endo Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 285: E1021-E1027, 2003. First published August 5, 2003; doi:10.1152/ajpendo.00234.2003
0193-1849/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/5/E1021    most recent
00234.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hildebrandt, A. L.
Right arrow Articles by Neufer, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hildebrandt, A. L.
Right arrow Articles by Neufer, P. D.

Differential transcriptional activation of select metabolic genes in response to variations in exercise intensity and duration

Audrey L. Hildebrandt,1 Henriette Pilegaard,3 and P. Darrell Neufer1,2

1John B. Pierce Laboratory and 2Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06519; and 3Copenhagen Muscle Research Center and August Krogh Institute, University of Copenhagen, Copenhagen, Denmark

Submitted 21 May 2003 ; accepted in final form 28 July 2003

Cellular adaptations to endurance training are influenced by the intensity and duration of exercise. To examine the impact of exercise intensity and duration on the acute transcriptional regulation of metabolic genes in red (RG) and white (WG) gastrocnemius muscle, rats completed either low-intensity [~50% maximal O2 uptake (O2 max)] treadmill exercise (LIE) for 45 min, LIE for 180 min, or high-intensity (~75% O2 max) exercise (HIE) for 45 min. LIE for 45 min activated (P < 0.05) transcription of the pyruvate dehydrogenase kinase-4 (PDK4), uncoupling protein-3 (UCP3), heme oxygenase-1 (HO-1), and hexokinase II (HK II) genes in RG within 1 h after exercise. In WG, transcription of PDK4, UCP3, HKII, and lipoprotein lipase (LPL) was also induced, whereas transcription of the HO-1 gene did not change. In RG, extending LIE duration from 45 to 180 min elicited a similar activation of PDK4 and UCP3 (~15-fold) but a far greater increase in HO-1 (>30-fold) and HKII transcription (>25-fold). In WG, extending LIE for 180 min induced a much greater and prolonged (through 2- to 4-h recovery) activation of PDK4, UCP3 (both >200-fold), and HO-1 (>10-fold). HIE elicited a similar pattern of gene activation to LIE in both RG and WG, with the exception that HIE triggered >10-fold activation of HO-1 in WG. These data provide evidence that both the intensity and the duration of exercise affect the transcriptional regulation of metabolic genes in muscle in a fiber type-specific manner, possibly reflecting the relative stress imposed by the exercise bout.

endurance training; mitochondrial biogenesis; gene regulation


Address for reprint requests and other correspondence: P. D. Neufer, John B. Pierce Laboratory, 290 Congress Ave., New Haven, CT 06519 (E-mail: dneufer{at}jbpierce.org).




This article has been cited by other articles:


Home page
 J. Physiol.Home page
M. Hoene, R. Lehmann, A. M. Hennige, A. K. Pohl, H. U. Haring, E. D. Schleicher, and C. Weigert
Acute regulation of metabolic genes and insulin receptor substrates in the liver of mice by one single bout of treadmill exercise
J. Physiol., January 1, 2009; 587(1): 241 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. Jorquera, N. Juretic, E. Jaimovich, and N. Riveros
Membrane depolarization induces calcium-dependent upregulation of Hsp70 and Hmox-1 in skeletal muscle cells
Am J Physiol Cell Physiol, January 1, 2009; 297(3): C581 - C590.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. Magkos, Y. E. Tsekouras, K. I. Prentzas, K. N. Basioukas, S. G. Matsama, A. E. Yanni, S. A. Kavouras, and L. S. Sidossis
Acute exercise-induced changes in basal VLDL-triglyceride kinetics leading to hypotriglyceridemia manifest more readily after resistance than endurance exercise
J Appl Physiol, October 1, 2008; 105(4): 1228 - 1236.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
Z. T. Bloomgarden
Diabetes and Obesity: Part 1
Diabetes Care, December 1, 2007; 30(12): 3145 - 3151.
[Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
K. C Hansen, Z. Zhang, T. Gomez, A. K Adams, and D. A Schoeller
Exercise increases the proportion of fat utilization during short-term consumption of a high-fat diet
Am. J. Clinical Nutrition, January 1, 2007; 85(1): 109 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. J. Gibala, J. P. Little, M. van Essen, G. P. Wilkin, K. A. Burgomaster, A. Safdar, S. Raha, and M. A. Tarnopolsky
Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance
J. Physiol., September 15, 2006; 575(3): 901 - 911.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. N. Kraniou, D. Cameron-Smith, and M. Hargreaves
Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity
J Appl Physiol, September 1, 2006; 101(3): 934 - 937.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
E. E. Spangenburg, D. A. Brown, M. S. Johnson, and R. L. Moore
Exercise increases SOCS-3 expression in rat skeletal muscle: potential relationship to IL-6 expression
J. Physiol., May 1, 2006; 572(3): 839 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. R. Wende, J. M. Huss, P. J. Schaeffer, V. Giguere, and D. P. Kelly
PGC-1{alpha} Coactivates PDK4 Gene Expression via the Orphan Nuclear Receptor ERR{alpha}: a Mechanism for Transcriptional Control of Muscle Glucose Metabolism
Mol. Cell. Biol., December 15, 2005; 25(24): 10684 - 10694.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. L. Seachrist, C.-M. Loi, M. G. Evans, K. A. Criswell, and C. E. Rothwell
Roles of Exercise and Pharmacokinetics in Cerivastatin-Induced Skeletal Muscle Toxicity
Toxicol. Sci., December 1, 2005; 88(2): 551 - 561.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. E. Civitarese, M. K. C. Hesselink, A. P. Russell, E. Ravussin, and P. Schrauwen
Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes
Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1023 - E1029.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Shearer, T. E. Graham, D. S. Battram, D. L. Robinson, E. A. Richter, R. J. Wilson, and M. Bakovic
Glycogenin activity and mRNA expression in response to volitional exhaustion in human skeletal muscle
J Appl Physiol, September 1, 2005; 99(3): 957 - 962.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K De Bock, E. A Richter, A. P Russell, B. O Eijnde, W Derave, M Ramaekers, E Koninckx, B Leger, J Verhaeghe, and P Hespel
Exercise in the fasted state facilitates fibre type-specific intramyocellular lipid breakdown and stimulates glycogen resynthesis in humans
J. Physiol., April 15, 2005; 564(2): 649 - 660.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. P. Massett and B. C. Berk
Strain-dependent differences in responses to exercise training in inbred and hybrid mice
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R1006 - R1013.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. Coles, J. Litt, H. Hatta, and A. Bonen
Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle
J. Physiol., November 15, 2004; 561(1): 253 - 261.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. J. Watt, G. J. F. Heigenhauser, P. J. LeBlanc, J. G. Inglis, L. L. Spriet, and S. J. Peters
Rapid upregulation of pyruvate dehydrogenase kinase activity in human skeletal muscle during prolonged exercise
J Appl Physiol, October 1, 2004; 97(4): 1261 - 1267.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. M. Bailey, I. S. Young, J. McEneny, L. Lawrenson, J. Kim, J. Barden, and R. S. Richardson
Regulation of free radical outflow from an isolated muscle bed in exercising humans
Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1689 - H1699.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.