HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/4/E864    most recent 00001.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Johansson, B.-L. Articles by Pernow, J. Search for Related Content PubMed PubMed Citation Articles by Johansson, B.-L. Articles by Pernow, J.

C-peptide increases forearm blood flow in patients with type 1 diabetes via a nitric oxide-dependent mechanism

¹Section of Clinical Physiology, Department of Surgical Sciences and ²Section of Cardiology, Department of Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden

Submitted 2 January 2003 ; accepted in final form 4 June 2003

Proinsulin C-peptide has been shown to increase muscle blood flow in type 1 diabetic patients. The underlying mechanism is not fully understood. The aim of this study was to evaluate if the vasodilator effect of C-peptide is mediated by nitric oxide (NO). Eleven type 1 diabetic patients were studied two times and randomized to administration of intravenous and intra-arterial infusion of C-peptide or saline. Forearm blood flow (FBF) was measured by venous occlusion plethysmography during infusion of C-peptide or saline before, during, and after NO synthase (NOS) blockade. Endothelium-dependent and -independent vasodilatation was evaluated by administration of acetylcholine and sodium nitroprusside, respectively. FBF increased by 35% during intravenous C-peptide (P < 0.01) but not during saline infusion (–2%, not significant). NOS blockade resulted in a more pronounced reduction in FBF during intravenous C-peptide than during saline infusion (–41 vs. –26%, P < 0.05). Intra-arterial C-peptide failed to increase FBF during NOS blockade. However, when C-peptide was given after the recovery from NOS blockade, FBF rose by 30% (P < 0.001). The vasodilator effects of acetylcholine and nitroprusside were not influenced by C-peptide. It is concluded that the stimulatory effect of C-peptide on FBF in type 1 diabetic patients is mediated via the NO system and that C-peptide increases basal endothelial NO levels.

acetylcholine; insulin; N^G-monomethyl-L-arginine; sodium nitroprusside; venous occlusion plethysmography

This article has been cited by other articles:

				A. Basu, N. Charkoudian, W. Schrage, R. A. Rizza, R. Basu, and M. J. Joyner Beneficial effects of GLP-1 on endothelial function in humans: dampening by glyburide but not by glimepiride Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1289 - E1295. [Abstract] [Full Text] [PDF]

				E. Polska, J. Kolodjaschna, F. Berisha, M. M. Malec, C. Simader, M. Bayerle-Eder, M. Roden, and L. Schmetterer C-Peptide does not affect ocular blood flow in patients with type 1 diabetes. Diabetes Care, September 1, 2006; 29(9): 2034 - 2038. [Abstract] [Full Text] [PDF]

				T. Nystrom, M. K. Gutniak, Q. Zhang, F. Zhang, J. J. Holst, B. Ahren, and A. Sjoholm Effects of glucagon-like peptide-1 on endothelial function in type 2 diabetes patients with stable coronary artery disease Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1209 - E1215. [Abstract] [Full Text] [PDF]