HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Kondo, A. Articles by Togari, A. Search for Related Content PubMed PubMed Citation Articles by Kondo, A. Articles by Togari, A.

In vivo stimulation of sympathetic nervous system modulates osteoblastic activity in mouse calvaria

Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Nagoya 464-8650, Japan

Submitted 21 January 2003 ; accepted in final form 25 April 2003

Previously, we demonstrated that epinephrine induced the expression of interleukin (IL)-6 mRNA via -adrenoceptors in cultured human osteoblastic cells. IL-6 is well known to modulate bone metabolism by regulating the development and function of osteoclasts and osteoblasts. Recently, restraint stress and intracerebroventricular injection of lipopolysaccharide (LPS) have been reported to induce the expression of IL-6 mRNA in peripheral organs in mice in which expression is mediated by the activation of the sympathetic nervous system. To prove the physiological role of sympathetic nerves in bone metabolism in vivo, we examined by RT-PCR analysis the effects of restraint stress and intracerebroventricular injection of LPS on IL-6 mRNA expression in mouse calvaria. The expression of IL-6 mRNA in mouse calvaria was stimulated by either restraint stress (30 min) or intracerebroventricular injection of LPS (50 ng/mouse, 60 min). The treatment of mice with the neurotoxin 6-hydroxydopamine (6-OHDA, 100 mg · kg^-1 · day^-1 ip for 3 days) inhibited LPS (icv)-induced expression of IL-6 mRNA in their calvaria. The expression of IL-6 mRNA induced by the restraint stress was not influenced by 6-OHDA, which destroys noradrenergic nerve terminals. Furthermore, pretreatment with a -blocker, propranolol (15 or 25 mg/kg ip), inhibited both stress- and LPS-induced increases in the level of IL-6 mRNA, but pretreatment with an -blocker, phentolamine (5 mg/kg sc), did not inhibit them in mouse calvaria. In addition, treatment of calvaria with isoprenaline or norepinephrine increased IL-6 synthesis in the organ culture system. These results indicate that in vivo adrenergic stimulation modulates the osteoblastic activity in mouse calvaria via noradrenergic nerve terminals.

restraint stress; intracerebroventricular injection; interleukin-6; lipopolysaccharide; calvaria; sympathetic activity; osteoblast

This article has been cited by other articles:

				N. Bonnet, C. L. Benhamou, H. Beaupied, N. Laroche, L. Vico, E. Dolleans, and D. Courteix Doping dose of salbutamol and exercise: deleterious effect on cancellous and cortical bones in adult rats J Appl Physiol, April 1, 2007; 102(4): 1502 - 1509. [Abstract] [Full Text] [PDF]