AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 285: E454-E459, 2003. First published May 20, 2003; doi:10.1152/ajpendo.00470.2002
0193-1849/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/3/E454    most recent
00470.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brommage, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brommage, R.

Validation and calibration of DEXA body composition in mice

Robert Brommage

Department of Endocrinology, Lexicon Genetics, The Woodlands, Texas 77381

Submitted 30 October 2002 ; accepted in final form 14 May 2003

Validated methods of determining murine body composition are required for studies of obesity in mice. Dual-energy X-ray absorptiometry (DEXA) provides a noninvasive approach to assess body fat and lean tissue contents. Similar to DEXA analyses in other species, body fat measurements in mice show acceptable precision but suffer from poor accuracy. Because fat and lean tissues each contain various components, these inaccuracies likely result from selection of inappropriate calibration standards. Analysis of solvents showed that the PIXImus2 DEXA gave results consistent with theoretical calculations. Male mice weighing 26-60 g and having body fat percentages ranging from 3 to 49% were analyzed by both PIXImus2 DEXA and chemical carcass analysis. DEXA overestimated mouse fat content by an average of 3.3 g, and algorithms were generated to calculate body fat from both measured body fat values and the measured ratio of high- to low-energy X-ray attenuations. With calibration to mouse body fat content measured by carcass analysis, the PIXImus2 DEXA gives accurate body composition values in mice.

PIXImus; carcass analysis; obesity; dual-energy X-ray absorptiometry


Address for reprint requests and other correspondence: R. Brommage, Dept. of Endocrinology, Lexicon Genetics, 8800 Technology Forest Pl., The Woodlands, TX 77381 (E-mail: rbrommage{at}lexgen.com).




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Scherbarth, J. Rozman, M. Klingenspor, G. Brabant, and S. Steinlechner
Wheel running affects seasonal acclimatization of physiological and morphological traits in the Djungarian hamster (Phodopus sungorus)
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1368 - R1375.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. S. Chow, L. J. Greenlund, Y. W. Asmann, K. R. Short, S. K. McCrady, J. A. Levine, and K. S. Nair
Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function
J Appl Physiol, March 1, 2007; 102(3): 1078 - 1089.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. K. Phan, W. K. Chung, and R. L. Leibel
The mahoganoid mutation (Mgrn1md) improves insulin sensitivity in mice with mutations in the melanocortin signaling pathway independently of effects on adiposity
Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E611 - E620.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Bjorkqvist, A. Petersen, K. Bacos, J. Isaacs, P. Norlen, J. Gil, N. Popovic, F. Sundler, G. P. Bates, S. J. Tabrizi, et al.
Progressive alterations in the hypothalamic-pituitary-adrenal axis in the R6/2 transgenic mouse model of Huntington's disease
Hum. Mol. Genet., May 15, 2006; 15(10): 1713 - 1721.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
H.-K. Kim, C. Nelson-Dooley, M. A. Della-Fera, J.-Y. Yang, W. Zhang, J. Duan, D. L. Hartzell, M. W. Hamrick, and C. A. Baile
Genistein Decreases Food Intake, Body Weight, and Fat Pad Weight and Causes Adipose Tissue Apoptosis in Ovariectomized Female Mice
J. Nutr., February 1, 2006; 136(2): 409 - 414.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
E. Kokkotou, J. Y. Jeon, X. Wang, F. E. Marino, M. Carlson, D. J. Trombly, and E. Maratos-Flier
Mice with MCH ablation resist diet-induced obesity through strain-specific mechanisms
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2005; 289(1): R117 - R124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. A. Held, W. Cosme-Blanco, L. M. Difedele, E. L. Bonkowski, R. K. Menon, and L. A. Denson
Alterations in growth hormone receptor abundance regulate growth hormone signaling in murine obstructive cholestasis
Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G986 - G993.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.