AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 284: E351-E356, 2003. First published October 8, 2002; doi:10.1152/ajpendo.00354.2002
0193-1849/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/E351    most recent
00354.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martini, W. Z.
Right arrow Articles by Brunengraber, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martini, W. Z.
Right arrow Articles by Brunengraber, H.
Vol. 284, Issue 2, E351-E356, February 2003

Quantitative assessment of anaplerosis from propionate in pig heart in vivo

Wenjun Z. Martini1,2, William C. Stanley1,2, Hazel Huang1, Christine Des Rosiers3, Charles L. Hoppel4, and Henri Brunengraber2

Departments of 1 Physiology and Biophysics, 2 Nutrition, and 4 Pharmacology and Medicine, Case Western Reserve University, Cleveland, Ohio 44106-7139; and 3 Department of Nutrition, University of Montreal, Montreal, Quebec, Canada H3C 3J7

Normal cardiac metabolism requires continuous replenishment (anaplerosis) of catalytic intermediates of the citric acid cycle. Little is known about the quantitative aspects of propionate as a substrate of in vivo anaplerosis; therefore, we measured the rate of propionate entry into the citric acid cycle in hearts of anesthetized pigs. [U-13C3]propionate (0.25 mM) was infused in a coronary artery branch for 1 h via an extracorporeal perfusion circuit, and cardiac biopsies were analyzed for the mass isotopomer distribution of citric acid cycle intermediates. Infusion of propionate did not affect myocardial oxygen consumption, heart rate, or contractile function. In the infused territory, propionate infusion did not affect uptake of glucose and lactate but decreased free fatty acid uptake by one-half (P < 0.05). Propionate extraction and uptake were 57.4 ± 3.3% and 0.078 ± 0.009 µmol · min-1 · g-1. Anaplerosis from propionate, calculated from the mass isotopomer distribution of succinate, accounted for 8.9 ± 1.3% of the citric acid cycle flux. Propioylcarnitine release accounted for only 0.033 ± 0.002% of propionate uptake. Methylcitrate did not accumulate. Thus administration of a low concentration of propionate appears to be a convenient and safe way to boost anaplerosis in the heart.

tricarboxylic acid cycle; cataplerosis; mass isotopomer analysis; mass spectrometry


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
I. C. Okere, T. A. McElfresh, D. Z. Brunengraber, W. Martini, J. P. Sterk, H. Huang, M. P. Chandler, H. Brunengraber, and W. C. Stanley
Differential effects of heptanoate and hexanoate on myocardial citric acid cycle intermediates following ischemia-reperfusion
J Appl Physiol, January 1, 2006; 100(1): 76 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
W. C. Stanley, F. A. Recchia, and G. D. Lopaschuk
Myocardial Substrate Metabolism in the Normal and Failing Heart
Physiol Rev, July 1, 2005; 85(3): 1093 - 1129.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. Zhou, J. E. Salem, G. M. Saidel, W. C. Stanley, and M. E. Cabrera
Mechanistic model of cardiac energy metabolism predicts localization of glycolysis to cytosolic subdomain during ischemia
Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2400 - H2411.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
N. Sharma, I. C. Okere, D. Z. Brunengraber, T. A. McElfresh, K. L. King, J. P. Sterk, H. Huang, M. P. Chandler, and W. C. Stanley
Regulation of pyruvate dehydrogenase activity and citric acid cycle intermediates during high cardiac power generation
J. Physiol., January 15, 2005; 562(2): 593 - 603.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online