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Vol. 284, Issue 2, E302-E312, February 2003

Somatotropin-induced protein anabolism in hindquarters and portal-drained viscera of growing pigs

Jill A. Bush¹, Douglas G. Burrin¹, Agus Suryawan¹, Pamela M. J. O'Connor¹, Hanh V. Nguyen¹, Peter J. Reeds^1,, Norman C. Steele², Johannes B. Van Goudoever¹, and Teresa A. Davis¹

¹ United States Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030; and ² United States Department of Agriculture/Agricultural Research Service Growth Biology Laboratory, Beltsville, Maryland 20705

To differentiate the effect of somatotropin (ST) treatment on protein metabolism in the hindquarter (HQ) and portal-drained viscera (PDV), growing swine (n = 20) treated with ST (0 or 150 µg · kg¹ · day¹) for 7 days were infused intravenously with NaH¹³CO₃ and [²H₅]phenylalanine and enterally with [1-¹³C]phenylalanine while in the fed state. Arterial, portal venous, and vena cava whole blood samples, breath samples, and blood flow measurements were obtained for determination of tissue and whole body phenylalanine kinetics under steady-state conditions. In the fed state, ST treatment decreased whole body phenylalanine flux, oxidation, and protein degradation without altering protein synthesis, resulting in an improvement in whole body net protein balance. Blood flow to the HQ (+80%), but not to the PDV, was increased with ST treatment. In the HQ and PDV, ST increased phenylalanine uptake (+44 and +23%, respectively) and protein synthesis (+43 and +41%, respectively), with no effect on protein degradation. In ST-treated and control pigs, phenylalanine was oxidized in the PDV (34-43% of enteral and arterial sources) but not the HQ. In both treatment groups, dietary (40%) rather than arterial (10%) extraction of phenylalanine predominated in gut amino acid metabolism, whereas localized blood flow influenced HQ amino acid metabolism. The results indicate that ST increases protein anabolism in young, growing swine by increasing protein synthesis in the HQ and PDV, with no effect on protein degradation. Differing results between the whole body and the HQ and PDV suggest that the effect of ST treatment on protein metabolism is tissue specific.

growth hormone; protein synthesis; protein degradation; amino acid kinetics; muscle

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Deceased 13 August 2002.

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