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-cells
1 Department of Medicine, 2 The Howard Hughes Medical Institute, 3 Departments of Pathology, 4 Neurobiology, Pharmacology and Physiology, and 5 Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637; and 6 Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232
We have generated transgenic mice
that express green fluorescent protein (GFP) under the control of the
mouse insulin I gene promoter (MIP). The MIP-GFP mice develop normally
and are indistinguishable from control animals with respect to glucose
tolerance and pancreatic insulin content. Histological studies showed
that the MIP-GFP mice had normal islet architecture with coexpression
of insulin and GFP in the 
-cells of all islets. We observed GFP
expression in islets from embryonic day E13.5 through adulthood.
Studies of -cell function revealed no difference in glucose-induced
intracellular calcium mobilization between islets from transgenic and
control animals. We prepared single-cell suspensions from both isolated islets and whole pancreas from MIP-GFP-transgenic mice and sorted the
-cells by fluorescence-activated cell sorting based on their green
fluorescence. These studies showed that 2.4 ± 0.2%
(n = 6) of the cells in the pancreas of newborn (P1)
and 0.9 ± 0.1% (n = 5) of 8-wk-old mice were
-cells. The MIP-GFP-transgenic mouse may be a useful tool for
studying -cell biology in normal and diabetic animals.
insulin; diabetes
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