AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 283: E971-E979, 2002. First published July 9, 2002; doi:10.1152/ajpendo.00021.2002
0193-1849/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/5/E971    most recent
00021.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Itani, O. A.
Right arrow Articles by Thomas, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Itani, O. A.
Right arrow Articles by Thomas, C. P.
Vol. 283, Issue 5, E971-E979, November 2002

Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5'-flanking region

Omar A. Itani1, Kang Z. Liu1, Kristyn L. Cornish1, Jason R. Campbell1, and Christie P. Thomas1,2

1 Department of Internal Medicine, University of Iowa College of Medicine and the 2 Veterans Affairs Medical Center, Iowa City, Iowa 52246

In lung and collecting duct epithelia, glucocorticoid (GC)-stimulated Na+ transport is preceded by an increase in the protein kinase sgk1, which in turn regulates the activity of the epithelial Na+ channel (ENaC). We investigated the mechanism for GC-regulated human sgk1 expression in lung and renal epithelia. sgk1 mRNA was increased in these epithelia by GCs, and this was inhibited by actinomycin D and superinduced by cycloheximide, consistent with a transcriptional effect that did not require protein synthesis. To understand the basis for transcriptional regulation, the transcription initiation site was mapped and the 5'-flanking region cloned by PCR. A 3-kb fragment of the upstream region was coupled to luciferase and transfected into A549 cells. By deletion analysis, an imperfect GC response element (GRE) was identified that was necessary and sufficient for GC responsiveness. When tested with cell extracts, a specific protein recognized by an anti-GC receptor (GR) antibody bound the GRE in gel mobility shift assays. We conclude that GCs stimulate sgk1 expression in human epithelial cells via activation of a GRE in the 5'-flanking region of sgk1.

epithelial Na+ channel; glucocorticoid response element; corticosteroids; airway epithelia; gene transcription; gel mobility shift assays


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Otulakowski, W. Duan, S. Gandhi, and H. O'Brodovich
Steroid and Oxygen Effects on eIF4F Complex, mTOR, and ENaC Translation in Fetal Lung Epithelia
Am. J. Respir. Cell Mol. Biol., October 1, 2007; 37(4): 457 - 466.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
E. C. Bolton, A. Y. So, C. Chaivorapol, C. M. Haqq, H. Li, and K. R. Yamamoto
Cell- and gene-specific regulation of primary target genes by the androgen receptor
Genes & Dev., August 15, 2007; 21(16): 2005 - 2017.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Wang, H. Zhang, F. Lang, and C. C. Yun
Acute activation of NHE3 by dexamethasone correlates with activation of SGK1 and requires a functional glucocorticoid receptor
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C396 - C404.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
F. Lang, C. Bohmer, M. Palmada, G. Seebohm, N. Strutz-Seebohm, and V. Vallon
(Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms.
Physiol Rev, October 1, 2006; 86(4): 1151 - 1178.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Otulakowski, B. Rafii, M. Harris, and H. O'Brodovich
Oxygen and Glucocorticoids Modulate {alpha}ENaC mRNA Translation in Fetal Distal Lung Epithelium
Am. J. Respir. Cell Mol. Biol., February 1, 2006; 34(2): 204 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. P. Thomas, K. Z. Liu, and H. S. Vats
Medroxyprogesterone acetate binds the glucocorticoid receptor to stimulate {alpha}-ENaC and sgk1 expression in renal collecting duct epithelia
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F306 - F312.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. Ullrich, S. Berchtold, F. Ranta, G. Seebohm, G. Henke, A. Lupescu, A. F. Mack, C.-M. Chao, J. Su, R. Nitschke, et al.
Serum- and Glucocorticoid-Inducible Kinase 1 (SGK1) Mediates Glucocorticoid-Induced Inhibition of Insulin Secretion
Diabetes, April 1, 2005; 54(4): 1090 - 1099.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. P. Thomas, J. R. Campbell, P. J. Wright, and R. F. Husted
cAMP-stimulated Na+ transport in H441 distal lung epithelial cells: role of PKA, phosphatidylinositol 3-kinase, and sgk1
Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L843 - L851.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Itani, J. R. Campbell, J. Herrero, P. M. Snyder, and C. P. Thomas
Alternate promoters and variable splicing lead to hNedd4-2 isoforms with a C2 domain and varying number of WW domains
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F916 - F929.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online