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1 Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582; and 2 Division of Clinical Immunology, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Beppu 874-0919, Japan
Adipose differentiation-related protein
(ADRP) is a lipid droplet-associated protein that is expressed early
during adipose differentiation. The present study was undertaken to
reveal the role of ADRP in adipose differentiation. In murine
fibroblasts infected with green fluorescent protein (GFP)-ADRP fusion
protein expression adenovirus vector, confocal microscopic analysis
showed the number and size of lipid droplets apparently increased
comparing with those of control cells. Overexpressed GFP-ADRP were
mainly located at the surface of lipid droplets and appeared to be
"ring-shaped." Triacylglycerol content was also significantly
(P < 0.001) increased in GFP-ADRP-overexpressed cells
compared with control cells. ADRP-induced lipid accumulation did not
depend on adipocyte-specific gene induction, such as peroxisome
proliferator-activated receptor-
, lipoprotein lipase, or other
lipogenic genes, including acyl-CoA synthetase, fatty acid-binding
protein, and fatty acid transporter. In conclusion, ADRP stimulated
lipid accumulation and lipid droplet formation without induction of
other adipocyte-specific genes or other lipogenic genes in murine
fibroblasts. The detailed molecular mechanisms of ADRP on lipid
accumulation remain to be elucidated.
adipose differentiation-related protein; triacylglycerol; adipocyte
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