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Am J Physiol Endocrinol Metab 283: E178-E186, 2002. First published March 12, 2002; doi:10.1152/ajpendo.00404.2001
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Vol. 283, Issue 1, E178-E186, July 2002

Effects of endurance training on activity and expression of AMP-activated protein kinase isoforms in rat muscles

Paula E. Durante1,*, Kirsty J. Mustard1,*, Soo-Hyun Park2, William W. Winder2, and D. Grahame Hardie1

1 Division of Molecular Physiology, School of Life Sciences, Dundee University, Wellcome Trust Biocentre, Dundee, DD1 5EH Scotland, UK; and 2 Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah 84602

The effects of endurance training on the response of muscle AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) to moderate treadmill exercise were examined. In red quadriceps, there was a large activation of alpha 2-AMPK and inactivation of ACC in response to exercise. This response was greatly reduced after training, probably because of reduced metabolic stress. In white quadriceps, there were no effects of exercise on AMPK or ACC, but alpha 2-activity was higher after training because of increased phosphorylation of Thr172. In soleus, there were small increases in alpha 2-activity during exercise that were not affected by training. The expression of all seven AMPK subunit isoforms was also examined. The beta 2- and gamma 2-isoforms were most highly expressed in white quadriceps, and gamma 3 was expressed in red quadriceps and soleus. There was a threefold increase in expression of gamma 3 after training in red quadriceps only. Our results suggest that gamma 3 might have a special role in the adaptation to endurance exercise in muscles utilizing oxidative metabolism.

gamma 3-isoform; acetyl-coenzyme A carboxylase; GLUT4; muscle mitochondrial enzymes; muscle gene expression

*  These authors contributed equally to this study.




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