AJP - Endo Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 282: E982-E991, 2002. First published January 2, 2002; doi:10.1152/ajpendo.00347.2001
0193-1849/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/E982    most recent
00347.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arredouani, A.
Right arrow Articles by Gilon, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arredouani, A.
Right arrow Articles by Gilon, P.
Vol. 282, Issue 5, E982-E991, May 2002

Contribution of the endoplasmic reticulum to the glucose-induced [Ca2+]c response in mouse pancreatic islets

Abdelilah Arredouani, Jean-Claude Henquin, and Patrick Gilon

Unité d'Endocrinologie et Métabolisme, University of Louvain Faculty of Medicine, UCL 55.30, B-1200 Brussels, Belgium

Thapsigargin (TG), a blocker of Ca2+ uptake by the endoplasmic reticulum (ER), was used to evaluate the contribution of the organelle to the oscillations of cytosolic Ca2+ concentration ([Ca2+]c) induced by repetitive Ca2+ influx in mouse pancreatic beta -cells. Because TG depolarized the plasma membrane in the presence of glucose alone, extracellular K+ was alternated between 10 and 30 mM in the presence of diazoxide to impose membrane potential (MP) oscillations. In control islets, pulses of K+, mimicking regular MP oscillations elicited by 10 mM glucose, induced [Ca2+]c oscillations whose nadir remained higher than basal [Ca2+]c. Increasing the depolarization phase of the pulses while keeping their frequency constant (to mimic the effects of a further rise of the glucose concentration on MP) caused an upward shift of the nadir of [Ca2+]c oscillations that was reproduced by raising extracellular Ca2+ (to increase Ca2+ influx) without changing the pulse protocol. In TG-pretreated islets, the imposed [Ca2+]c oscillations were of much larger amplitude than in control islets and occurred on basal levels. During intermittent trains of depolarizations, control islets displayed mixed [Ca2+]c oscillations characterized by a summation of fast oscillations on top of slow ones, whereas no progressive summation of the fast oscillations was observed in TG-pretreated islets. In conclusion, the buffering capacity of the ER in pancreatic beta -cells limits the amplitude of [Ca2+]c oscillations and may explain how the nadir between oscillations remains above baseline during regular oscillations or gradually increases during mixed [Ca2+]c oscillations, two types of response observed during glucose stimulation.

cytosolic Ca2+ concentration oscillations; endoplasmic reticulum; pancreatic beta -cell; thapsigargin


This article has been cited by other articles:


Home page
 J EndocrinolHome page
G. F Anhe, T. C A Nogueira, J. E Nicoletti-Carvalho, C. Lellis-Santos, H. C Barbosa, J. Cipolla-Neto, J. R Bosqueiro, A. C Boschero, and S. Bordin
Signal transducer and activator of transcription 3-regulated sarcoendoplasmic reticulum Ca2+-ATPase 2 expression by prolactin and glucocorticoids is involved in the adaptation of insulin secretory response during the peripartum period
J. Endocrinol., October 1, 2007; 195(1): 17 - 27.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. C. Beauvois, C. Merezak, J.-C. Jonas, M. A. Ravier, J.-C. Henquin, and P. Gilon
Glucose-induced mixed [Ca2+]c oscillations in mouse beta-cells are controlled by the membrane potential and the SERCA3 Ca2+-ATPase of the endoplasmic reticulum
Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1503 - C1511.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
N. A. Tamarina, A. Kuznetsov, C. J. Rhodes, V. P. Bindokas, and L. H. Philipson
Inositol (1,4,5)-Trisphosphate Dynamics and Intracellular Calcium Oscillations in Pancreatic {beta}-Cells
Diabetes, November 1, 2005; 54(11): 3073 - 3081.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. C. Beauvois, A. Arredouani, J.-C. Jonas, J.-F. Rolland, F. Schuit, J.-C. Henquin, and P. Gilon
Atypical Ca2+-induced Ca2+ release from a sarco-endoplasmic reticulum Ca2+-ATPase 3-dependent Ca2+ pool in mouse pancreatic {beta}-cells
J. Physiol., August 15, 2004; 559(1): 141 - 156.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online