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Am J Physiol Endocrinol Metab 281: E1101-E1109, 2001;
0193-1849/01 $5.00
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Vol. 281, Issue 5, E1101-E1109, November 2001

Glucose uptake and glucose transporter proteins in skeletal muscle from undernourished rats

María Agote, Luis Goya, Sonia Ramos, Carmen Alvarez, M. Lucía Gavete, Ana M. Pascual-Leone, and Fernando Escrivá

Instituto de Bioquímica, Centro Mixto Consejo Superior Investigaciones Científicas Universidad Complutense de Madrid, Facultad Farmacia, Universidad Complutense, Ciudad Universitaria, 28040 Madrid, Spain

Undernutrition in rats impairs secretion of insulin but maintains glucose normotolerance, because muscle tissue presents an increased insulin-induced glucose uptake. We studied glucose transporters in gastrocnemius muscles from food-restricted and control anesthetized rats under basal and euglycemic hyperinsulinemic conditions. Muscle membranes were prepared by subcellular fractionation in sucrose gradients. Insulin-induced glucose uptake, estimated by a 2-deoxyglucose technique, was increased 4- and 12-fold in control and food-restricted rats, respectively. Muscle insulin receptor was increased, but phosphotyrosine-associated phosphatidylinositol 3-kinase activity stimulated by insulin was lower in undernourished rats, whereas insulin receptor substrate-1 content remained unaltered. The main glucose transporter in the muscle, GLUT-4, was severely reduced albeit more efficiently translocated in response to insulin in food-deprived rats. GLUT-1, GLUT-3, and GLUT-5, minor isoforms in skeletal muscle, were found increased in food-deprived rats. The rise in these minor glucose carriers, as well as the improvement in GLUT-4 recruitment, is probably insufficient to account for the insulin-induced increase in the uptake of glucose in undernourished rats, thereby suggesting possible changes in other steps required for glucose metabolism.

undernutrition; muscle glucose transporters; insulin signaling


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