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Am J Physiol Endocrinol Metab 281: E1037-E1044, 2001;
0193-1849/01 $5.00
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Vol. 281, Issue 5, E1037-E1044, November 2001

Human adipose tissue endothelial cells promote preadipocyte proliferation

L. J. Hutley1, A. C. Herington4, W. Shurety1, C. Cheung2, D. A. Vesey2, D. P. Cameron3, and J. B. Prins1

1 Department of Diabetes and Endocrinology and University of Queensland Department of Medicine, 2 Department of Nephrology, and 3 Princess Alexandra Hospital Centres for Health Research, Princess Alexandra Hospital, Woolloongabba, Queensland 4102; and 4 Centre for Molecular Biotechnology, School of Life Sciences, Gardens Point Campus, Queensland University of Technology, Brisbane, Queensland, Australia 4001

Adipogenesis is preceded by development of a microvascular network, and optimal functioning of adipose tissue as an energy store and endocrine organ is dependent on extensive vascularization. We have examined the role of endothelial cell-derived factors that influence the proliferation of human preadipocytes. Microvascular endothelial cells and preadipocytes were isolated from human omental and subcutaneous adipose tissue biopsies by use of a developed procedure of collagenase digest, immunoselection, and differential trypsinization. Conditioned medium from microvascular endothelial cell cultures promoted the proliferation of preadipocytes (P = <0.001) and (to a lesser extent) other cell types. No depot-specific differences in mitogenic capacity of microvascular endothelial cell medium or of preadipocyte response were observed. These results indicate that adipose tissue endothelial cells secrete soluble adipogenic factor(s).

adipose-derived microvascular endothelial cells; isolation and purification techniques; paracrine interactions; conditioned media; proliferation assay


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