AJP - Endo Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 281: E242-E247, 2001;
0193-1849/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brandt, A.
Right arrow Articles by Byrne, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brandt, A.
Right arrow Articles by Byrne, M. M.
Vol. 281, Issue 2, E242-E247, August 2001

GLP-1-induced alterations in the glucose-stimulated insulin secretory dose-response curve

Andreas Brandt1, Martin Katschinski1, Rudolf Arnold1, Kenneth S. Polonsky2, Burkhard Göke3, and Maria M. Byrne1

1 The Clinical Research Unit for Gastrointestinal Endocrinology, Department of Internal Medicine, Philipps University, 35033 Marburg, Germany; 2 Department of Internal Medicine, Washington University, St. Louis, Missouri 63110; and 3 Department of Gastroenterology, Inselspital, University of Bern, CH-3010 Bern, Switzerland

The present study was undertaken to establish in normal volunteers the alterations in beta -cell responsiveness to glucose associated with a constant infusion of glucagon-like peptide-1 (GLP-1) or a pretreatment infusion for 60 min. A high-dose graded glucose infusion protocol was used to explore the dose-response relationship between glucose and insulin secretion. Studies were performed in 10 normal volunteers, and insulin secretion rates (ISR) were calculated by deconvolution of peripheral C-peptide levels by use of a two-compartmental model that utilized mean kinetic parameters. During the saline study, from 5 to 15 mM glucose, the relationship between glucose and ISR was linear. Constant GLP-1 infusion (0.4 pmol · kg-1 · min-1) shifted the dose-response curve to the left, with an increase in the slope of this curve from 5 to 9 mM glucose from 71.0 ± 12.4 pmol · min-1 · mM-1 during the saline study to 241.7 ± 36.6 pmol · min-1 · mM-1 during the constant GLP-1 infusion (P < 0.0001). GLP-1 consistently stimulated a >200% increase in ISR at each 1 mM glucose interval, maintaining plasma glucose at <10 mM (P < 0.0007). Pretreatment with GLP-1 for 60 min resulted in no significant priming of the beta -cell response to glucose (P = 0.2). Insulin clearance rates were similar in all three studies at corresponding insulin levels. These studies demonstrate that physiological levels of GLP-1 stimulate glucose-induced insulin secretion in a linear manner, with a consistent increase in ISR at each 1 mM glucose interval, and that they have no independent effect on insulin clearance and no priming effect on subsequent insulin secretory response to glucose.

insulin secretion; connecting peptide; priming; beta -cell sensitivity


This article has been cited by other articles:


Home page
 DiabetesHome page
D. S. Edgerton, K. M.S. Johnson, D. W. Neal, M. Scott, C. H. Hobbs, X. Zhang, A. Duttaroy, and A. D. Cherrington
Inhibition of Dipeptidyl Peptidase-4 by Vildagliptin During Glucagon-Like Peptide 1 Infusion Increases Liver Glucose Uptake in the Conscious Dog
Diabetes, January 1, 2009; 58(1): 243 - 249.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. J. Meier, J. J. Holst, W. E. Schmidt, and M. A. Nauck
Reduction of hepatic insulin clearance after oral glucose ingestion is not mediated by glucagon-like peptide 1 or gastric inhibitory polypeptide in humans
Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E849 - E856.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. Muscelli, A. Mari, A. Natali, B. D. Astiarraga, S. Camastra, S. Frascerra, J. J. Holst, and E. Ferrannini
Impact of incretin hormones on beta-cell function in subjects with normal or impaired glucose tolerance
Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1144 - E1150.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
N. N. Rudovich, H. J. Rochlitz, and A. F.H. Pfeiffer
Reduced Hepatic Insulin Extraction in Response to Gastric Inhibitory Polypeptide Compensates for Reduced Insulin Secretion in Normal-Weight and Normal Glucose Tolerant First-Degree Relatives of Type 2 Diabetic Patients
Diabetes, September 1, 2004; 53(9): 2359 - 2365.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. A. Hinke, K. Hellemans, and F. C. Schuit
Plasticity of the {beta} cell insulin secretory competence: preparing the pancreatic {beta} cell for the next meal
J. Physiol., July 15, 2004; 558(2): 369 - 380.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
S. Quddusi, T. P. Vahl, K. Hanson, R. L. Prigeon, and D. A. D'Alessio
Differential Effects of Acute and Extended Infusions of Glucagon-Like Peptide-1 on First- and Second-Phase Insulin Secretion in Diabetic and Nondiabetic Humans
Diabetes Care, March 1, 2003; 26(3): 791 - 798.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. L. Kjems, J. J. Holst, A. Volund, and S. Madsbad
The Influence of GLP-1 on Glucose-Stimulated Insulin Secretion: Effects on {beta}-Cell Sensitivity in Type 2 and Nondiabetic Subjects
Diabetes, February 1, 2003; 52(2): 380 - 386.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online