AJP - Endo Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 281: E16-E24, 2001;
0193-1849/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shefi-Friedman, L.
Right arrow Articles by Sampson, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shefi-Friedman, L.
Right arrow Articles by Sampson, S. R.
Vol. 281, Issue 1, E16-E24, July 2001

Increased IGFR activity and glucose transport in cultured skeletal muscle from insulin receptor null mice

Liat Shefi-Friedman1, Efrat Wertheimer2, Shlomzion Shen1, Asia Bak1, Domenico Accili3, and Sanford R. Sampson1

1 Gonda-Goldschmeid Diagnostic Center, Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900; 2 Department of Pathology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv 69978, Israel; and 3 Columbia University/ Berrie Research Pavilion, New York, New York 10032

We have studied the role of the insulin receptor (IR) in metabolic and growth-promoting effects of insulin on primary cultures of skeletal muscle derived from the limb muscle of IR null mice. Cultures of IR null skeletal muscle displayed normal morphology and spontaneous contractile activity. Expression of muscle-differentiating proteins was slightly reduced in myoblasts and myotubes of the IR null skeletal muscle cells, whereas that of the Na+/K+ pump appeared to be unchanged. Insulin-like growth factor receptor (IGFR) expression was higher in myoblasts from IR knockout (IRKO) than from IR wild-type (IRWT) mice but was essentially unchanged in myotubes. Expression of the GLUT-1 and GLUT-4 transporters appeared to be higher in IRKO than in IRWT myoblasts and was significantly greater in myotubes from IRKO than from IRWT cultures. Consistent with GLUT expression, both basal and insulin or insulin-like growth factor I (IGF-I)-stimulated glucose uptakes were higher in IR null skeletal myotubes than in wild-type skeletal myotubes. Interestingly, autophosphorylation of IGFR induced by insulin and IGF-I was markedly increased in IR null skeletal myotubes. These results indicate that, in the absence of IR, there is a compensatory increase in basal as well as in insulin- and IGF-I-induced glucose transport, the former being mediated via increased activation of the IGF-I receptor.

myoblasts; myotubes; insulin receptor; insulin-like growth factor I receptor; GLUT; insulin receptor null mouse


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
F. M. Smith, L. J. Holt, A. S. Garfield, M. Charalambous, F. Koumanov, M. Perry, R. Bazzani, S. A. Sheardown, B. D. Hegarty, R. J. Lyons, et al.
Mice with a Disruption of the Imprinted Grb10 Gene Exhibit Altered Body Composition, Glucose Homeostasis, and Insulin Signaling during Postnatal Life
Mol. Cell. Biol., August 15, 2007; 27(16): 5871 - 5886.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
P. Zheng, R. Vassena, and K. E. Latham
Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos
Mol. Hum. Reprod., June 1, 2007; 13(6): 361 - 371.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. G. Laustsen, S. J. Russell, L. Cui, A. Entingh-Pearsall, M. Holzenberger, R. Liao, and C. R. Kahn
Essential Role of Insulin and Insulin-Like Growth Factor 1 Receptor Signaling in Cardiac Development and Function
Mol. Cell. Biol., March 1, 2007; 27(5): 1649 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. C. Manara, L. Landuzzi, P. Nanni, G. Nicoletti, D. Zambelli, P. L. Lollini, C. Nanni, F. Hofmann, C. Garcia-Echeverria, P. Picci, et al.
Preclinical In vivo Study of New Insulin-Like Growth Factor-I Receptor-Specific Inhibitor in Ewing's Sarcoma
Clin. Cancer Res., February 15, 2007; 13(4): 1322 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. Carvalho, K. Kotani, O. D. Peroni, and B. B. Kahn
Adipose-specific overexpression of GLUT4 reverses insulin resistance and diabetes in mice lacking GLUT4 selectively in muscle
Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E551 - E561.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. NANDI, Y. KITAMURA, C. R. KAHN, and D. ACCILI
Mouse Models of Insulin Resistance
Physiol Rev, April 1, 2004; 84(2): 623 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. L. Hribal, F. Oriente, and D. Accili
Mouse models of insulin resistance
Am J Physiol Endocrinol Metab, May 1, 2002; 282(5): E977 - E981.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online