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, andPhysical Activity Sciences Laboratory, Division of Kinesiology, Department of Social and Preventive Medicine, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada G1K 7P4
To determine the
effects of weight loss on intramyocellular energy substrates, vastus
lateralis muscle biopsies were taken from six obese subjects (body mass
index 34 ± 5 kg/m2) before, after 15 wk of energy
restriction (ER; 
700 kcal/day), and after a further average 20.7 ± 1.6 wk of endurance training plus low-fat diet (ET-LFD). Body weight
fell from 100 ± 6 to 89 ± 6 kg during ER and to 84 ± 4 kg after ET-LFD. Lipids and glycogen were histochemically measured in
type I, IIA, and IIB fibers. Total muscle glycogen content (MGC; per
100 fibers) decreased after ER [from 72 ± 13 to 55 ± 8 arbitrary units (AU)]. A similar but not significant decrease was seen
in total muscle lipid content (MLC; 14 ± 5 to 9 ± 1 AU).
After ET-LFD, MGC returned to initial values (74 ± 8 AU), and MLC
approached near-initial values (12 ± 3 AU). Individual fiber
lipid concentration did not change throughout the protocol in all fiber
types, whereas glycogen concentration increased after ET-LFD. The
training effects of ET-LFD were measured as increasing activities of
key mitochondrial enzymes. Although total muscle energy reserves can be
reduced after weight loss, their concentration within individual
myofibers remains elevated. Weight loss does not appear sufficient to
correct the potential detrimental effects of high intracellular lipid concentrations.
obesity; weight reduction; triglyceride; lipid metabolism; Oil red O; glycogen
Deceased 27 August, 1999
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