| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Nutritional Sciences, Rutgers University and 2 Saint Peter's University Hospital, New Brunswick, New Jersey 08901
The objective of this study was to determine whether obese human adipose tissue contains preformed stores of leptin and their relationship to secreted leptin. Detergent increased detectable leptin by about twofold, suggesting that leptin is stored in a membrane-bound location. Subcutaneous tissue leptin was ~1.6-fold higher than omental, paralleling known differences in leptin secretion and expression. The amount of leptin secreted during a 3-h incubation was similar to that of extractable tissue leptin. Tissue leptin levels were maintained over the incubation. Inhibition of protein synthesis decreased tissue leptin content but did not decrease leptin secretion until after 3 h of incubation. Culture of adipose tissue for 2 days with the combination of insulin and dexamethasone, but not with either hormone alone, increased tissue leptin content about twofold in both depots. Although insulin did not affect tissue leptin content, it potentiated leptin secretion (as a % of tissue stores). These data suggest that adipose tissue leptin storage and secretion per se are regulated. Regulation of the release of preformed leptin may modulate serum leptin levels in obese humans.
secretion; insulin; dexamethasone; organ culture; cycloheximide
This article has been cited by other articles:
|
|
 |
|
  M.-J. Lee, Y. Wang, M. R. Ricci, S. Sullivan, C. D. Russell, and S. K. Fried Acute and chronic regulation of leptin synthesis, storage, and secretion by insulin and dexamethasone in human adipose tissue Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E858 - E864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-J. Lee and S. K. Fried Multilevel regulation of leptin storage, turnover, and secretion by feeding and insulin in rat adipose tissue J. Lipid Res., September 1, 2006; 47(9): 1984 - 1993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Lynch, B. Gern, C. Lloyd, S. M. Hutson, R. Eicher, and T. C. Vary Leucine in food mediates some of the postprandial rise in plasma leptin concentrations Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E621 - E630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Ricci, M.-J. Lee, C. D. Russell, Y. Wang, S. Sullivan, S. H. Schneider, R. E. Brolin, and S. K. Fried Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms Am J Physiol Endocrinol Metab, April 1, 2005; 288(4): E798 - E804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tabe, M. Konopleva, M. F. Munsell, F. C. Marini, C. Zompetta, T. McQueen, T. Tsao, S. Zhao, S. Pierce, J. Igari, et al. PML-RAR{alpha} is associated with leptin-receptor induction: the role of mesenchymal stem cell-derived adipocytes in APL cell survival Blood, March 1, 2004; 103(5): 1815 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Johnson, J. B. Albu, E. S. Engelson, S. K. Fried, Y. Inada, G. Ionescu, and D. P. Kotler Increased systemic and adipose tissue cytokines in patients with HIV-associated lipodystrophy Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E261 - E271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Roh, J. Han, A. Tzatsos, and K. V. Kandror Nutrient-sensing mTOR-mediated pathway regulates leptin production in isolated rat adipocytes Am J Physiol Endocrinol Metab, February 1, 2003; 284(2): E322 - E330. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
