AJP - Endo AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 280: E301-E307, 2001;
0193-1849/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rasmussen, U. F.
Right arrow Articles by Bangsbo, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rasmussen, U. F.
Right arrow Articles by Bangsbo, J.
Vol. 280, Issue 2, E301-E307, February 2001

Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria

Ulla F. Rasmussen1, Hans N. Rasmussen1, Peter Krustrup2, Bjørn Quistorff3, Bengt Saltin4, and Jens Bangsbo2

1 Department of Biochemistry, August Krogh Institute, University of Copenhagen; 2 Copenhagen Muscle Research Center, Institute of Exercise and Sport Sciences, University of Copenhagen; 3 Nuclear Magnetic Resonance Center, Panum Institute, University of Copenhagen; and 4 Copenhagen Muscle Research Center, Rigshospitalet, Copenhagen DK-2100, Denmark

The aim of the present study was to examine whether parameters of isolated mitochondria could account for the in vivo maximum oxygen uptake (VO2 max) of human skeletal muscle. VO2 max and work performance of the quadriceps muscle of six volunteers were measured in the knee extensor model (range 10-18 mmol O2 · min-1 · kg-1 at work rates of 22-32 W/kg). Mitochondria were isolated from the same muscle at rest. Strong correlations were obtained between VO2 max and a number of mitochondrial parameters (mitochondrial protein, cytochrome aa3, citrate synthase, and respiratory activities). The activities of citrate synthase, succinate dehydrogenase, and pyruvate dehydrogenase, measured in isolated mitochondria, corresponded to, respectively, 15, 3, and 1.1 times the rates calculated from VO2 max. The respiratory chain activity also appeared sufficient. Fully coupled in vitro respiration, which is limited by the rate of ATP synthesis, could account for, at most, 60% of the VO2 max. This might be due to systematic errors or to loose coupling of the mitochondrial respiration under intense exercise.

skeletal muscles; maximal oxygen uptake; work rate; respiration; adenosine triphosphate synthesis


This article has been cited by other articles:


Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
P. A. Figueiredo, R. M. Ferreira, H. J. Appell, and J. A. Duarte
Age-Induced Morphological, Biochemical, and Functional Alterations in Isolated Mitochondria From Murine Skeletal Muscle
J. Gerontol. A Biol. Sci. Med. Sci., April 1, 2008; 63(4): 350 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Sahlin, M. Mogensen, M. Bagger, M. Fernstrom, and P. K. Pedersen
The potential for mitochondrial fat oxidation in human skeletal muscle influences whole body fat oxidation during low-intensity exercise
Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E223 - E230.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
B. L. Pedersen, H. Arendrup, N. H. Secher, and B. Quistorff
Metabolism of perfused pig intercostal muscles evaluated by 31P-magnetic resonance spectroscopy
Exp Physiol, July 1, 2006; 91(4): 755 - 763.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
H. K. LEE, K. S. PARK, Y. M. CHO, Y. Y. LEE, and Y. K. PAK
The role of the mitochondria in human aging and disease: from gene to cell signaling. Proceedings of the second scientific meeting of the Asian Society for Mitochondrial Research and Medicine. April 1-2, 2004. Taipei, Taiwan.
Ann. N.Y. Acad. Sci., May 1, 2005; 1042: 1 - 537.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
V. Bezaire, G. J. F. Heigenhauser, and L. L. Spriet
Regulation of CPT I activity in intermyofibrillar and subsarcolemmal mitochondria from human and rat skeletal muscle
Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E85 - E91.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online