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Am J Physiol Endocrinol Metab 280: E301-E307, 2001;
0193-1849/01 $5.00
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Vol. 280, Issue 2, E301-E307, February 2001

Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria

Ulla F. Rasmussen1, Hans N. Rasmussen1, Peter Krustrup2, Bjørn Quistorff3, Bengt Saltin4, and Jens Bangsbo2

1 Department of Biochemistry, August Krogh Institute, University of Copenhagen; 2 Copenhagen Muscle Research Center, Institute of Exercise and Sport Sciences, University of Copenhagen; 3 Nuclear Magnetic Resonance Center, Panum Institute, University of Copenhagen; and 4 Copenhagen Muscle Research Center, Rigshospitalet, Copenhagen DK-2100, Denmark

The aim of the present study was to examine whether parameters of isolated mitochondria could account for the in vivo maximum oxygen uptake (VO2 max) of human skeletal muscle. VO2 max and work performance of the quadriceps muscle of six volunteers were measured in the knee extensor model (range 10-18 mmol O2 · min-1 · kg-1 at work rates of 22-32 W/kg). Mitochondria were isolated from the same muscle at rest. Strong correlations were obtained between VO2 max and a number of mitochondrial parameters (mitochondrial protein, cytochrome aa3, citrate synthase, and respiratory activities). The activities of citrate synthase, succinate dehydrogenase, and pyruvate dehydrogenase, measured in isolated mitochondria, corresponded to, respectively, 15, 3, and 1.1 times the rates calculated from VO2 max. The respiratory chain activity also appeared sufficient. Fully coupled in vitro respiration, which is limited by the rate of ATP synthesis, could account for, at most, 60% of the VO2 max. This might be due to systematic errors or to loose coupling of the mitochondrial respiration under intense exercise.

skeletal muscles; maximal oxygen uptake; work rate; respiration; adenosine triphosphate synthesis


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