AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 280: E296-E300, 2001;
0193-1849/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Novel-Chaté, V.
Right arrow Articles by Tappy, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Novel-Chaté, V.
Right arrow Articles by Tappy, L.
Vol. 280, Issue 2, E296-E300, February 2001

Role of Na+-K+-ATPase in insulin-induced lactate release by skeletal muscle

Valérie Novel-Chaté1, Valentine Rey1, René Chioléro2, Philippe Schneiter1, Xavier Leverve3, Eric Jéquier1, and Luc Tappy1

1 Institute of Physiology, Medical School, University of Lausanne, 1005; 2 Surgical Intensive Care Unit, University Hospital, 1011 Lausanne, Switzerland; and 3 Laboratory of Fundamental and Applied Bioenergetics, University Joseph Fourier, 38041 Grenoble, France

Hyperinsulinemia increases lactate release by various organs and tissues. Whereas it has been shown that aerobic glycolysis is linked to Na+-K+-ATPase activity, we hypothesized that stimulation by insulin of skeletal muscle Na+-K+-ATPase is responsible for increased muscle lactate production. To test this hypothesis, we assessed muscle lactate release in healthy volunteers from the [13C]lactate concentration in the effluent dialysates of microdialysis probes inserted into the tibialis anterior muscles on both sides and infused with solutions containing 5 mmol/l [U-13C]glucose. On one side, the microdialysis probe was intermittently infused with the same solution additioned with 2.10-5 M ouabain. In the basal state, [13C]lactate concentration in the dialysate was not affected by ouabain. During a euglycemic-hyperinsulinemic clamp, [13C]lactate concentration increased by 135% in the dialysate without ouabain, and this stimulation was nearly entirely reversed by ouabain (56% inhibition compared with values in the dialysate collected from the contralateral probe). These data indicate that insulin stimulates muscle lactate release by activating Na+-K+-ATPase in healthy humans.

aerobic glycolysis; [13C]lactate; microdialysis; ouabain


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
V. Qvisth, E. Hagstrom-Toft, E. Moberg, S. Sjoberg, and J. Bolinder
Lactate release from adipose tissue and skeletal muscle in vivo: defective insulin regulation in insulin-resistant obese women
Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E709 - E714.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Bundgaard, K. Kjeldsen, K. Suarez Krabbe, G. van Hall, L. Simonsen, J. Qvist, C. Muff Hansen, K. Moller, L. Fonsmark, P. Lav Madsen, et al.
Endotoxemia stimulates skeletal muscle Na+-K+-ATPase and raises blood lactate under aerobic conditions in humans
Am J Physiol Heart Circ Physiol, March 1, 2003; 284(3): H1028 - H1034.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Okamoto, W. Wang, J. Rounds, E. A. Chambers, and D. O. Jacobs
ATP from glycolysis is required for normal sodium homeostasis in resting fast-twitch rodent skeletal muscle
Am J Physiol Endocrinol Metab, September 1, 2001; 281(3): E479 - E488.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online