AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 280: E221-E228, 2001;
0193-1849/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (78)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Taylor, W. E.
Right arrow Articles by Gonzalez-Cadavid, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taylor, W. E.
Right arrow Articles by Gonzalez-Cadavid, N.
Vol. 280, Issue 2, E221-E228, February 2001

Myostatin inhibits cell proliferation and protein synthesis in C2C12 muscle cells

Wayne E. Taylor1, Shalender Bhasin1, Jorge Artaza1, Frances Byhower1, Mohd Azam2, Darril H. Willard Jr.3, Frederick C. Kull Jr.3, and Nestor Gonzalez-Cadavid1

1 Division of Endocrinology, Metabolism and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059; 2 ExpressGen, Chicago, Illinois 60612; 3 Glaxo-Wellcome Laboratories, Research Triangle Park, North Carolina 27709

Myostatin mutations in mice and cattle are associated with increased muscularity, suggesting that myostatin is a negative regulator of skeletal muscle mass. To test the hypothesis that myostatin inhibits muscle cell growth, we examined the effects of recombinant myostatin in mouse skeletal muscle C2C12 cells. After verification of the expression of cDNA constructs in a cell-free system and in transfected Chinese hamster ovary cells, the human recombinant protein was expressed as the full-length (375-amino acid) myostatin in Drosophila cells (Mst375D), or the 110-amino acid carboxy-terminal protein in Escherichia coli (Mst110EC). These proteins were identified by immunoblotting and were purified. Both Mst375D and Mst110EC dose dependently inhibited cell proliferation (cell count and Formazan assay), DNA synthesis ([3H]thymidine incorporation), and protein synthesis ([1-14C]leucine incorporation) in C2C12 cells. The inhibitory effects of both proteins were greater in myotubes than in myoblasts. Neither protein had any significant effects on protein degradation or apoptosis. In conclusion, recombinant myostatin proteins inhibit cell proliferation, DNA synthesis, and protein synthesis in C2C12 muscle cells, suggesting that myostatin may control muscle mass by inhibiting muscle growth or regeneration.

myoblast; myotube; growth differentiation factor 8; sarcopenia; growth differentiation factor


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. L. Allen, A. S. Cleary, K. J. Speaker, S. F. Lindsay, J. Uyenishi, J. M. Reed, M. C. Madden, and R. S. Mehan
Myostatin, activin receptor IIb, and follistatin-like-3 gene expression are altered in adipose tissue and skeletal muscle of obese mice
Am J Physiol Endocrinol Metab, May 1, 2008; 294(5): E918 - E927.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
O Schakman, H Gilson, and J P Thissen
Mechanisms of glucocorticoid-induced myopathy
J. Endocrinol., April 1, 2008; 197(1): 1 - 10.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. Manceau, J. Gros, K. Savage, V. Thome, A. McPherron, B. Paterson, and C. Marcelle
Myostatin promotes the terminal differentiation of embryonic muscle progenitors
Genes & Dev., March 1, 2008; 22(5): 668 - 681.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
J. N Artaza, R. Singh, M. G Ferrini, M. Braga, J. Tsao, and N. F Gonzalez-Cadavid
Myostatin promotes a fibrotic phenotypic switch in multipotent C3H 10T1/2 cells without affecting their differentiation into myofibroblasts
J. Endocrinol., February 1, 2008; 196(2): 235 - 249.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J.-s. Kim, J. K. Petrella, J. M. Cross, and M. M. Bamman
Load-mediated downregulation of myostatin mRNA is not sufficient to promote myofiber hypertrophy in humans: a cluster analysis
J Appl Physiol, November 1, 2007; 103(5): 1488 - 1495.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. Antony, J. J. Bass, C. D. McMahon, and M. D. Mitchell
Myostatin regulates glucose uptake in BeWo cells
Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1296 - E1302.
[Abstract] [Full Text] [PDF]

Home page
 Poult. Sci.Home page
Y. S. Kim, N. K. Bobbili, Y. K. Lee, H. J. Jin, and M. A. Dunn
Production of a Polyclonal Anti-Myostatin Antibody and the Effects of In Ovo Administration of the Antibody on Posthatch Broiler Growth and Muscle Mass
Poult. Sci., June 1, 2007; 86(6): 1196 - 1205.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
G. McKoy, K. A. Bicknell, K. Patel, and G. Brooks
Developmental expression of myostatin in cardiomyocytes and its effect on foetal and neonatal rat cardiomyocyte proliferation
Cardiovasc Res, May 1, 2007; 74(2): 304 - 312.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. M. Heinemeier, J. L. Olesen, P. Schjerling, F. Haddad, H. Langberg, K. M. Baldwin, and M. Kjaer
Short-term strength training and the expression of myostatin and IGF-I isoforms in rat muscle and tendon: differential effects of specific contraction types
J Appl Physiol, February 1, 2007; 102(2): 573 - 581.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. L. Allen and T. G. Unterman
Regulation of myostatin expression and myoblast differentiation by FoxO and SMAD transcription factors
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C188 - C199.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. L. Mendias, J. E. Marcin, D. R. Calerdon, and J. A. Faulkner
Contractile properties of EDL and soleus muscles of myostatin-deficient mice
J Appl Physiol, September 1, 2006; 101(3): 898 - 905.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. D. Kollias, R. L. S. Perry, T. Miyake, A. Aziz, and J. C. McDermott
Smad7 promotes and enhances skeletal muscle differentiation.
Mol. Cell. Biol., August 1, 2006; 26(16): 6248 - 6260.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Welle, K. Bhatt, and C. A. Pinkert
Myofibrillar protein synthesis in myostatin-deficient mice
Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E409 - E415.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
J. G. Marblestone, S. C. Edavettal, Y. Lim, P. Lim, X. Zuo, and T. R. Butt
Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO
Protein Sci., January 1, 2006; 15(1): 182 - 189.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J.-s. Kim, J. M. Cross, and M. M. Bamman
Impact of resistance loading on myostatin expression and cell cycle regulation in young and older men and women
Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1110 - E1119.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. W. Jackman and S. C. Kandarian
The molecular basis of skeletal muscle atrophy
Am J Physiol Cell Physiol, October 1, 2004; 287(4): C834 - C843.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. H. Lang, R. A. Frost, E. Svanberg, and T. C. Vary
IGF-I/IGFBP-3 ameliorates alterations in protein synthesis, eIF4E availability, and myostatin in alcohol-fed rats
Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E916 - E926.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
W. Huygens, M. A. Thomis, M. W. Peeters, J. Aerssens, R. Janssen, R. F. Vlietinck, and G. Beunen
Linkage of myostatin pathway genes with knee strength in humans
Physiol Genomics, May 19, 2004; 17(3): 264 - 270.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. B. P. CHARGE and M. A. RUDNICKI
Cellular and Molecular Regulation of Muscle Regeneration
Physiol Rev, January 1, 2004; 84(1): 209 - 238.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C. Xu, G. Wu, Y. Zohar, and S.-J. Du
Analysis of myostatin gene structure, expression and function in zebrafish
J. Exp. Biol., November 15, 2003; 206(22): 4067 - 4079.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
K. E. Yarasheski
Review Article: Exercise, Aging, and Muscle Protein Metabolism
J. Gerontol. A Biol. Sci. Med. Sci., October 1, 2003; 58(10): M918 - 922.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Reisz-Porszasz, S. Bhasin, J. N. Artaza, R. Shen, I. Sinha-Hikim, A. Hogue, T. J. Fielder, and N. F. Gonzalez-Cadavid
Lower skeletal muscle mass in male transgenic mice with muscle-specific overexpression of myostatin
Am J Physiol Endocrinol Metab, October 1, 2003; 285(4): E876 - E888.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. McCroskery, M. Thomas, L. Maxwell, M. Sharma, and R. Kambadur
Myostatin negatively regulates satellite cell activation and self-renewal
J. Cell Biol., September 15, 2003; 162(6): 1135 - 1147.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Ma, C. Mallidis, S. Bhasin, V. Mahabadi, J. Artaza, N. Gonzalez-Cadavid, J. Arias, and B. Salehian
Glucocorticoid-induced skeletal muscle atrophy is associated with upregulation of myostatin gene expression
Am J Physiol Endocrinol Metab, August 1, 2003; 285(2): E363 - E371.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. D. McMahon, L. Popovic, J. M. Oldham, F. Jeanplong, H. K. Smith, R. Kambadur, M. Sharma, L. Maxwell, and J. J. Bass
Myostatin-deficient mice lose more skeletal muscle mass than wild-type controls during hindlimb suspension
Am J Physiol Endocrinol Metab, July 1, 2003; 285(1): E82 - E87.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. D. McMahon, L. Popovic, F. Jeanplong, J. M. Oldham, S. P. Kirk, C. C. Osepchook, K. W. Y. Wong, M. Sharma, R. Kambadur, and J. J. Bass
Sexual dimorphism is associated with decreased expression of processed myostatin in males
Am J Physiol Endocrinol Metab, February 1, 2003; 284(2): E377 - E381.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. J. Marcell, S. M. Harman, R. J. Urban, D. D. Metz, B. D. Rodgers, and M. R. Blackman
Comparison of GH, IGF-I, and testosterone with mRNA of receptors and myostatin in skeletal muscle in older men
Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1159 - E1164.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Rios, I. Carneiro, V. M. Arce, and J. Devesa
Myostatin is an inhibitor of myogenic differentiation
Am J Physiol Cell Physiol, May 1, 2002; 282(5): C993 - C999.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online