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1 Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Clermont-Ferrand-Theix, 63122 Saint Genès Champanelle, France; and 2 Centre de Recherches Nestlé, Vers chez les blanc, Lausanne 26, Switzerland
Methionine transsulfuration in plasma and liver, and plasma
methionine and cysteine kinetics were investigated in vivo during the
acute phase of sepsis in rats. Rats were infected with an intravenous
injection of live Escherichia coli, and control pair-fed rats were injected with saline. Two days after injection, the rats were
infused for 6 h with [35S]methionine and
[15N]cysteine. Transsulfuration was measured from the
transfer rate of 35S from methionine to cysteine. Liver
cystathionase activity was also measured. Infection significantly
increased (P < 0.05) the contribution of
transsulfuration to cysteine flux in both plasma and liver (by 80%)
and the contribution of transsulfuration to plasma methionine flux (by
133%). Transsulfuration measured in plasma was significantly
(P < 0.05) higher in infected rats than in pair-fed
rats (0.68 and 0.25 µmol · h
1 · 100 g1, respectively). However, liver cystathionase specific
activity was decreased by 17% by infection (P < 0.05). Infection increased methionine flux (16%, P < 0.05) less than cysteine flux (38%, P < 0.05).
Therefore, the plasma cysteine flux was higher than that predicted from
estimates of protein turnover based on methionine data, probably
because of enhanced glutathione turnover. Taken together, these results
suggest an increased cysteine requirement in infection.
methionine flux; cystathionase; components of cysteine flux
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