AJP - Endo Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 279: E1358-E1365, 2000;
0193-1849/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, B.
Right arrow Articles by Nagy, L. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, B.
Right arrow Articles by Nagy, L. E.
Vol. 279, Issue 6, E1358-E1365, December 2000

Ethanol stimulates glucose uptake and translocation of GLUT-4 in H9c2 myotubes via a Ca2+-dependent mechanism

Bo Yu, Adrienne Schroeder, and Laura E. Nagy

Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106

Short-term exposure to ethanol impairs glucose homeostasis, but the effects of ethanol on individual components of the glucose disposal pathway are not known. To understand the mechanisms by which ethanol disrupts glucose homeostasis, we have investigated the direct effects of ethanol on glucose uptake and translocation of GLUT-4 in H9c2 myotubes. Short-term treatment with 12.5-50 mM ethanol increased uptake of 2-deoxyglucose by 1.8-fold in differentiated myotubes. Pretreatment of H9c2 myotubes with 100 nM wortmannin, an inhibitor of phosphatidylinositol 3-kinase, had no effect on ethanol-induced increases in 2-deoxyglucose uptake. In contrast, preincubation with 25 µM dantrolene, an inhibitor of Ca2+ release from the sarcoplasmic reticulum, blocked the stimulation of 2-deoxyglucose uptake by ethanol. Increased 2-deoxyglucose uptake after ethanol treatment was associated with a decrease in small intracellular GLUT-4 vesicles and an increase in GLUT-4 localized at the cell surface. In contrast, ethanol had no effect on the quantity of GLUT-1 and GLUT-3 at the plasma membrane. These data demonstrate that physiologically relevant concentrations of ethanol disrupt the trafficking of GLUT-4 in H9c2 myotubes resulting in translocation of GLUT-4 to the plasma membrane and increased glucose uptake.

skeletal muscle; glucose tolerance; insulin; phosphatidylinositol 3-kinase; vesicle trafficking


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Saeedi, V. V. Saran, S. S. Y. Wu, E. S. Kume, K. Paulson, A. P. K. Chan, H. L. Parsons, R. B. Wambolt, J. R. B. Dyck, R. W. Brownsey, et al.
AMP-activated protein kinase influences metabolic remodeling in H9c2 cells hypertrophied by arginine vasopressin
Am J Physiol Heart Circ Physiol, June 1, 2009; 296(6): H1822 - H1832.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Saeedi, H. L. Parsons, R. B. Wambolt, K. Paulson, V. Sharma, J. R. B. Dyck, R. W. Brownsey, and M. F. Allard
Metabolic actions of metformin in the heart can occur by AMPK-independent mechanisms
Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2497 - H2506.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
E. R. Gross, A. K. Hsu, and G. J. Gross
Diabetes Abolishes Morphine-Induced Cardioprotection via Multiple Pathways Upstream of Glycogen Synthase Kinase-3{beta}
Diabetes, January 1, 2007; 56(1): 127 - 136.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. Cai, W. Li, G. Wang, L. Guo, Y. Jiang, and Y. J. Kang
Hyperglycemia-Induced Apoptosis in Mouse Myocardium: Mitochondrial Cytochrome c-Mediated Caspase-3 Activation Pathway
Diabetes, June 1, 2002; 51(6): 1938 - 1948.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online