| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Biochemistry and 2 Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214
Artificial rearing of neonatal rats on a high-carbohydrate (HC) milk formula resulted in the immediate onset of hyperinsulinemia. This study examines, in islets of 12-day-old HC rats, adaptive changes that support the hyperinsulinemic state. Increases in plasma glucagon-like peptide-1 (GLP-1) levels and islet GLP-1 receptor mRNA supported increased insulin secretion by HC islets. Isolated HC islets, but not mother-fed (MF) islets, secreted moderate amounts of insulin in a glucose- and Ca2+-independent manner. Under stringent Ca2+-free conditions and in the presence of glucose, GLP-1 plus acetylcholine augmented insulin release to a larger extent in HC islets. Levels of adenylyl cyclase type VI mRNA and activities of protein kinase A, protein kinase C, and calcium calmodulin kinase II were increased in HC islets. A tenfold increase in norepinephrine concentration was required to inhibit insulin secretion in HC islets compared with MF islets, indicating reduced sensitivity to adrenergic signals. This study shows that significant alterations at proximal and distal sites of the insulin secretory pathway in HC islets may support the hyperinsulinemic state of these rats.
hyperinsulinemia; nutritional intervention; protein kinase C; protein kinase A; glucagon-like peptide-1; adenylyl cyclase
This article has been cited by other articles:
|
|
 |
|
  M. Srinivasan, P. Mitrani, G. Sadhanandan, C. Dodds, S. Shbeir-ElDika, S. Thamotharan, H. Ghanim, P. Dandona, S. U Devaskar, and M. S Patel A high-carbohydrate diet in the immediate postnatal life of rats induces adaptations predisposing to adult-onset obesity J. Endocrinol., June 1, 2008; 197(3): 565 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mitrani, M. Srinivasan, C. Dodds, and M. S. Patel Autonomic involvement in the permanent metabolic programming of hyperinsulinemia in the high-carbohydrate rat model Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1364 - E1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mitrani, M. Srinivasan, C. Dodds, and M. S. Patel Role of the autonomic nervous system in the development of hyperinsulinemia by high-carbohydrate formula feeding to neonatal rats Am J Physiol Endocrinol Metab, April 1, 2007; 292(4): E1069 - E1078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Cerf, K. Williams, X. I. Nkomo, C. J. Muller, D. F. Du Toit, J. Louw, and S. A. Wolfe-Coote Islet cell response in the neonatal rat after exposure to a high-fat diet during pregnancy Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1122 - R1128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Srinivasan, R. Aalinkeel, F. Song, and M. S. Patel Programming of Islet Functions in the Progeny of Hyperinsulinemic/Obese Rats Diabetes, April 1, 2003; 52(4): 984 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tian and S. G. Laychock Protein Kinase C and Calcium Regulation of Adenylyl Cyclase in Isolated Rat Pancreatic Islets Diabetes, November 1, 2001; 50(11): 2505 - 2513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Aalinkeel, M. Srinivasan, F. Song, and M. S. Patel Programming into adulthood of islet adaptations induced by early nutritional intervention in the rat Am J Physiol Endocrinol Metab, September 1, 2001; 281(3): E640 - E648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Song, M. Srinivasan, R. Aalinkeel, and M. S. Patel Use of a cDNA Array for the Identification of Genes Induced in Islets of Suckling Rats by a High-Carbohydrate Nutritional Intervention Diabetes, September 1, 2001; 50(9): 2053 - 2060. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
