| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Pediatrics, United States Department of Agriculture/Agricultural Research Service, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030; and 2 Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033
The rapid gain in skeletal muscle mass in the neonate is associated with a marked elevation in skeletal muscle protein synthesis in response to feeding. The feeding-induced response decreases with development. To determine whether the response to feeding is regulated at the level of translation initiation, the expression, phosphorylation, and function of a number of eukaryotic initiation factors (eIF) were examined. Pigs at 7 and 26 days of age were either fasted overnight or fed porcine milk after an overnight fast. In muscle of 7-day-old pigs, the hyperphosphorylated form of the eIF4E repressor protein, 4E-binding protein 1 (4E-BP1), was undetectable in the fasting state but rose to 60% of total 4E-BP1 after feeding; eIF4E phosphorylation was unaffected by feeding status. The amount of eIF4E in the inactive 4E-BP1 · eIF4E complex was reduced by 80%, and the amount of eIF4E in the active eIF4E · eIF4G complex was increased 14-fold in muscle of 7-day-old pigs after feeding. The amount of 70-kDa ribosomal protein S6 (p70S6) kinase in the hyperphosphorylated form rose 2.5-fold in muscle of 7-day-old pigs after feeding. Each of these feeding-induced responses was blunted in muscle of 26-day-old pigs. eIF2B activity in muscle was unaffected by feeding status but decreased with development. Feeding produced similar changes in eIF characteristics in liver and muscle; however, the developmental changes in liver were not as apparent as in skeletal muscle. Thus the results demonstrate that the developmental change in the acute stimulation of skeletal muscle protein synthesis by feeding is regulated by the availability of eIF4E for 48S ribosomal complex formation. The results further suggest that the overall developmental decline in skeletal muscle protein synthesis involves regulation by eIF2B.
protein synthesis; growth; insulin; eukaryotic initiation factor 4E; liver
This article has been cited by other articles:
|
|
 |
|
  F. A. Wilson, R. A. Orellana, A. Suryawan, H. V. Nguyen, A. S. Jeyapalan, J. Frank, and T. A. Davis Stimulation of muscle protein synthesis by somatotropin in pigs is independent of the somatotropin-induced increase in circulating insulin Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E187 - E194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yao, Y.-L. Yin, W. Chu, Z. Liu, D. Deng, T. Li, R. Huang, J. Zhang, B. Tan, W. Wang, et al. Dietary Arginine Supplementation Increases mTOR Signaling Activity in Skeletal Muscle of Neonatal Pigs J. Nutr., May 1, 2008; 138(5): 867 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Escobar, J. W. Frank, A. Suryawan, H. V. Nguyen, and T. A. Davis Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1615 - E1621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suryawan, R. A. Orellana, H. V. Nguyen, A. S. Jeyapalan, J. R. Fleming, and T. A. Davis Activation by insulin and amino acids of signaling components leading to translation initiation in skeletal muscle of neonatal pigs is developmentally regulated Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1597 - E1605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Orellana, A. Jeyapalan, J. Escobar, J. W. Frank, H. V. Nguyen, A. Suryawan, and T. A. Davis Amino acids augment muscle protein synthesis in neonatal pigs during acute endotoxemia by stimulating mTOR-dependent translation initiation Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1416 - E1425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Jeyapalan, R. A. Orellana, A. Suryawan, P. M. J. O'Connor, H. V. Nguyen, J. Escobar, J. W. Frank, and T. A. Davis Glucose stimulates protein synthesis in skeletal muscle of neonatal pigs through an AMPK- and mTOR-independent process Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E595 - E603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Orellana, S. R. Kimball, A. Suryawan, J. Escobar, H. V. Nguyen, L. S. Jefferson, and T. A. Davis Insulin stimulates muscle protein synthesis in neonates during endotoxemia despite repression of translation initiation Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E629 - E636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suryawan, J. Escobar, J. W. Frank, H. V. Nguyen, and T. A. Davis Developmental regulation of the activation of signaling components leading to translation initiation in skeletal muscle of neonatal pigs Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E849 - E859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Rhoads, X. Niu, J. Odle, and L. M. Graves Role of mTOR signaling in intestinal cell migration Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G510 - G517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Orellana, P. M. J. O'Connor, J. A. Bush, A. Suryawan, M. C. Thivierge, H. V. Nguyen, M. L. Fiorotto, and T. A. Davis Modulation of muscle protein synthesis by insulin is maintained during neonatal endotoxemia Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E159 - E166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Caso, P. J. Garlick, L. M. Ballou, J. A. Vosswinkel, M. C. Gelato, and M. A. McNurlan The Increase in Human Muscle Protein Synthesis Induced by Food Intake Is Similar When Assessed with the Constant Infusion and Flooding Techniques J. Nutr., June 1, 2006; 136(6): 1504 - 1510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Escobar, J. W. Frank, A. Suryawan, H. V. Nguyen, S. R. Kimball, L. S. Jefferson, and T. A. Davis Regulation of cardiac and skeletal muscle protein synthesis by individual branched-chain amino acids in neonatal pigs Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E612 - E621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Frank, J. Escobar, A. Suryawan, H. V. Nguyen, S. R. Kimball, L. S. Jefferson, and T. A. Davis Dietary protein and lactose increase translation initiation factor activation and tissue protein synthesis in neonatal pigs Am J Physiol Endocrinol Metab, February 1, 2006; 290(2): E225 - E233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Escobar, J. W. Frank, A. Suryawan, H. V. Nguyen, S. R. Kimball, L. S. Jefferson, and T. A. Davis Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation Am J Physiol Endocrinol Metab, May 1, 2005; 288(5): E914 - E921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Sans, S.-H. Lee, L. G. D'Alecy, and J. A. Williams Feeding activates protein synthesis in mouse pancreas at the translational level without increase in mRNA Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G667 - G675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. J. O'Connor, S. R. Kimball, A. Suryawan, J. A. Bush, H. V. Nguyen, L. S. Jefferson, and T. A. Davis Regulation of neonatal liver protein synthesis by insulin and amino acids in pigs Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E994 - E1003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Davis, J. A. Bush, R. C. Vann, A. Suryawan, S. R. Kimball, and D. G. Burrin Somatotropin regulation of protein metabolism in pigs J Anim Sci, January 1, 2004; 82(13_suppl): E207 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. J. O'Connor, S. R. Kimball, A. Suryawan, J. A. Bush, H. V. Nguyen, L. S. Jefferson, and T. A. Davis Regulation of translation initiation by insulin and amino acids in skeletal muscle of neonatal pigs Am J Physiol Endocrinol Metab, July 1, 2003; 285(1): E40 - E53. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Jefferson and S. R. Kimball Amino Acids as Regulators of Gene Expression at the Level of mRNA Translation J. Nutr., June 1, 2003; 133(6): 2046S - 2051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suryawan and T. A. Davis Protein-tyrosine-phosphatase 1B activation is regulated developmentally in muscle of neonatal pigs Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E47 - E54. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Davis, M. L. Fiorotto, D. G. Burrin, R. C. Vann, P. J. Reeds, H. V. Nguyen, P. R. Beckett, and J. A. Bush Acute IGF-I infusion stimulates protein synthesis in skeletal muscle and other tissues of neonatal pigs Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E638 - E647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Kimball, P. A. Farrell, and L. S. Jefferson Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Role of insulin in translational control of protein synthesis in skeletal muscle by amino acids or exercise J Appl Physiol, September 1, 2002; 93(3): 1168 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Shen, D. Mallon, D. W. Boyle, and E. A. Liechty IGF-I and insulin regulate eIF4F formation by different mechanisms in muscle and liver in the ovine fetus Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E593 - E603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Kimball Regulation of Global and Specific mRNA Translation by Amino Acids J. Nutr., May 1, 2002; 132(5): 883 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suryawan, H. V. Nguyen, J. A. Bush, and T. A. Davis Developmental changes in the feeding-induced activation of the insulin-signaling pathway in neonatal pigs Am J Physiol Endocrinol Metab, November 1, 2001; 281(5): E908 - E915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Davis, M. L. Fiorotto, P. R. Beckett, D. G. Burrin, P. J. Reeds, D. Wray-Cahen, and H. V. Nguyen Differential effects of insulin on peripheral and visceral tissue protein synthesis in neonatal pigs Am J Physiol Endocrinol Metab, May 1, 2001; 280(5): E770 - E779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Kimball, P. A. Farrell, H. V. Nguyen, L. S. Jefferson, and T. A. Davis Developmental decline in components of signal transduction pathways regulating protein synthesis in pig muscle Am J Physiol Endocrinol Metab, March 1, 2002; 282(3): E585 - E592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Davis, M. L. Fiorotto, D. G. Burrin, P. J. Reeds, H. V. Nguyen, P. R. Beckett, R. C. Vann, and P. M. J. O'Connor Stimulation of protein synthesis by both insulin and amino acids is unique to skeletal muscle in neonatal pigs Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E880 - E890. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
